Constitutive L-Sox5 overexpression delays differentiation of ATDC5 cells into chondrocytes and correlates with reduced expression of differentiation markers
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Date
Authors
Sun, Dongmei
Deepak, Vishwa
Mu, Ping
Jiang, Haiying
Shi, Xiuming
Liu, Zhongbo
Zeng, Xianlu
Liu, Wenguang
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
L-Sox5 is a member of sex-determining region
Y-type high mobility group box (SOX) family of transcription
factors. We assessed the effects of retroviral
overexpression of L-Sox5 on chondrocyte differentiation
using the clonal murine cell line ATDC5. We observed a
temporal-restricted expression pattern of L-Sox5 in insulininduced
ATDC5 cells differentiating toward chondrocyte
lineage. The protein expression levels of L-Sox5 showed a
drastic decrease in contrast to unaltered mRNA levels
during differentiation. L-Sox5 delayed the differentiation
of ATDC5 cells as evidenced by Alcian blue staining for
proteoglycan synthesis. The mRNA levels of chondrocyte
and hypertrophic/osteoarthritic markers were markedly
decreased or delayed in L-Sox5 overexpressing cells.
L-Sox5 abrogated the promoter activity of Runx2. These
results suggest that L-Sox5 protein expression may
diminish along with the progress of chondrogenic differentiation. L-Sox5 may act as a negative regulator if
expressed aberrantly at least in part by regulating the
critical fate of chondrogenesis.
Description
Keywords
L-Sox5, Chondrogenesis, Runx2, Hypertrophy, Asporin, Osteoarthritis
Sustainable Development Goals
Citation
Sun, D, Deepak, V, Mu, P, Jiang, H, Shi, X, Liu, Z, Zeng, X & Liu, W 2015, 'Constitutive L-Sox5 overexpression delays differentiation of ATDC5 cells into chondrocytes and correlates with reduced expression of differentiation markers', Molecular and Cellular Biochemistry, vol. 401, no. 1-2, pp. 21-26.