dc.contributor.author |
Sun, Dongmei
|
|
dc.contributor.author |
Deepak, Vishwa
|
|
dc.contributor.author |
Mu, Ping
|
|
dc.contributor.author |
Jiang, Haiying
|
|
dc.contributor.author |
Shi, Xiuming
|
|
dc.contributor.author |
Liu, Zhongbo
|
|
dc.contributor.author |
Zeng, Xianlu
|
|
dc.contributor.author |
Liu, Wenguang
|
|
dc.date.accessioned |
2016-02-12T10:06:19Z |
|
dc.date.issued |
2015-03 |
|
dc.description.abstract |
L-Sox5 is a member of sex-determining region
Y-type high mobility group box (SOX) family of transcription
factors. We assessed the effects of retroviral
overexpression of L-Sox5 on chondrocyte differentiation
using the clonal murine cell line ATDC5. We observed a
temporal-restricted expression pattern of L-Sox5 in insulininduced
ATDC5 cells differentiating toward chondrocyte
lineage. The protein expression levels of L-Sox5 showed a
drastic decrease in contrast to unaltered mRNA levels
during differentiation. L-Sox5 delayed the differentiation
of ATDC5 cells as evidenced by Alcian blue staining for
proteoglycan synthesis. The mRNA levels of chondrocyte
and hypertrophic/osteoarthritic markers were markedly
decreased or delayed in L-Sox5 overexpressing cells.
L-Sox5 abrogated the promoter activity of Runx2. These
results suggest that L-Sox5 protein expression may
diminish along with the progress of chondrogenic differentiation. L-Sox5 may act as a negative regulator if
expressed aberrantly at least in part by regulating the
critical fate of chondrogenesis. |
en_ZA |
dc.description.embargo |
2016-03-31 |
|
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
National Natural Science Foundation of China (30270660), the Jilin Province Science and Technology Development Project (20040114), the Fok Ying Tung Education Foundation (91025), and the Training Fund of NENU’S Scientific Innovation Project NENUSTC08015). |
en_ZA |
dc.description.uri |
http://link.springer.com/journal/11010 |
en_ZA |
dc.identifier.citation |
Sun, D, Deepak, V, Mu, P, Jiang, H, Shi, X, Liu, Z, Zeng, X & Liu, W 2015, 'Constitutive L-Sox5 overexpression delays differentiation of ATDC5 cells into chondrocytes and correlates with reduced expression of differentiation markers', Molecular and Cellular Biochemistry, vol. 401, no. 1-2, pp. 21-26. |
en_ZA |
dc.identifier.issn |
0300-8177 (print) |
|
dc.identifier.issn |
1573-4919 (online) |
|
dc.identifier.other |
10.1007/s11010-014-2288-8 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/51344 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Springer |
en_ZA |
dc.rights |
© Springer Science+Business Media New York 2014. The original publication is available at : http://link.springer.comjournal/11010. |
en_ZA |
dc.subject |
L-Sox5 |
en_ZA |
dc.subject |
Chondrogenesis |
en_ZA |
dc.subject |
Runx2 |
en_ZA |
dc.subject |
Hypertrophy |
en_ZA |
dc.subject |
Asporin |
en_ZA |
dc.subject |
Osteoarthritis |
en_ZA |
dc.title |
Constitutive L-Sox5 overexpression delays differentiation of ATDC5 cells into chondrocytes and correlates with reduced expression of differentiation markers |
en_ZA |
dc.type |
Postprint Article |
en_ZA |