Constitutive L-Sox5 overexpression delays differentiation of ATDC5 cells into chondrocytes and correlates with reduced expression of differentiation markers

dc.contributor.authorSun, Dongmei
dc.contributor.authorDeepak, Vishwa
dc.contributor.authorMu, Ping
dc.contributor.authorJiang, Haiying
dc.contributor.authorShi, Xiuming
dc.contributor.authorLiu, Zhongbo
dc.contributor.authorZeng, Xianlu
dc.contributor.authorLiu, Wenguang
dc.contributor.emailvishwa.deepak@up.ac.zaen_ZA
dc.date.accessioned2016-02-12T10:06:19Z
dc.date.issued2015-03
dc.description.abstractL-Sox5 is a member of sex-determining region Y-type high mobility group box (SOX) family of transcription factors. We assessed the effects of retroviral overexpression of L-Sox5 on chondrocyte differentiation using the clonal murine cell line ATDC5. We observed a temporal-restricted expression pattern of L-Sox5 in insulininduced ATDC5 cells differentiating toward chondrocyte lineage. The protein expression levels of L-Sox5 showed a drastic decrease in contrast to unaltered mRNA levels during differentiation. L-Sox5 delayed the differentiation of ATDC5 cells as evidenced by Alcian blue staining for proteoglycan synthesis. The mRNA levels of chondrocyte and hypertrophic/osteoarthritic markers were markedly decreased or delayed in L-Sox5 overexpressing cells. L-Sox5 abrogated the promoter activity of Runx2. These results suggest that L-Sox5 protein expression may diminish along with the progress of chondrogenic differentiation. L-Sox5 may act as a negative regulator if expressed aberrantly at least in part by regulating the critical fate of chondrogenesis.en_ZA
dc.description.embargo2016-03-31
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipNational Natural Science Foundation of China (30270660), the Jilin Province Science and Technology Development Project (20040114), the Fok Ying Tung Education Foundation (91025), and the Training Fund of NENU’S Scientific Innovation Project NENUSTC08015).en_ZA
dc.description.urihttp://link.springer.com/journal/11010en_ZA
dc.identifier.citationSun, D, Deepak, V, Mu, P, Jiang, H, Shi, X, Liu, Z, Zeng, X & Liu, W 2015, 'Constitutive L-Sox5 overexpression delays differentiation of ATDC5 cells into chondrocytes and correlates with reduced expression of differentiation markers', Molecular and Cellular Biochemistry, vol. 401, no. 1-2, pp. 21-26.en_ZA
dc.identifier.issn0300-8177 (print)
dc.identifier.issn1573-4919 (online)
dc.identifier.other10.1007/s11010-014-2288-8
dc.identifier.urihttp://hdl.handle.net/2263/51344
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer Science+Business Media New York 2014. The original publication is available at : http://link.springer.comjournal/11010.en_ZA
dc.subjectL-Sox5en_ZA
dc.subjectChondrogenesisen_ZA
dc.subjectRunx2en_ZA
dc.subjectHypertrophyen_ZA
dc.subjectAsporinen_ZA
dc.subjectOsteoarthritisen_ZA
dc.titleConstitutive L-Sox5 overexpression delays differentiation of ATDC5 cells into chondrocytes and correlates with reduced expression of differentiation markersen_ZA
dc.typePostprint Articleen_ZA

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