dc.contributor.author |
Slabbert, Marisa
|
|
dc.contributor.author |
Du Plessis, S.S.
|
|
dc.contributor.author |
Huyser, Carin
|
|
dc.date.accessioned |
2015-12-14T05:18:47Z |
|
dc.date.issued |
2015-06 |
|
dc.description.abstract |
Vitrification is a simple and cost-effective method for the storage of human spermatozoa without the use of conventional
cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. As a result, solidification of living cells
without the formation of ice crystals is achieved during cooling. This study aimed to compare cryoprotectant-free
vitrification to conventional cryopreservation protocols. Semen samples (n = 35) were collected from patients seeking
diagnostic assistance at the Reproductive and Endocrine Unit at Steve Biko Academic Hospital. Samples were processed
using a discontinu-ous density-gradient centrifugation method. Washed samples were split into two aliquots and
cryopreserved either by means of cryoprotectant-free vitrifica-tion (sucrose + 1% albumin) or conventional slow freezing
(TEST-yolk buf-fer). Post-thawing, the sperm motion parameters, mitochondrial membrane potential (Dwm) and DNA
fragmentation were compared between the two groups. No significant differences were observed in the sperm motility
parame-ters (P > 0.05). Significantly higher percentages of Dwm (11.99% 4.326%versus 6.58% 1.026%; P < 0.001) and
lower percentages of DNA fragmenta-tion (2.79% 1.017% versus 3.86% 1.38%; P < 0.01) were observed when
comparing cryoprotectant-free vitrification to conventional cryopreservation. Cryoprotectant-free vitrification is a rapid and
promising alternative to conventional methods resulting in good-quality spermatozoa post-thaw. |
en_ZA |
dc.description.embargo |
2016-06-30 |
|
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
Research Committee of the Faculty of Health Sciences (RESCOM), University of Pretoria,
Merck Serono (Pty) Ltd and the National Research Foundation. |
en_ZA |
dc.description.uri |
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0272 |
en_ZA |
dc.identifier.citation |
Slabbert, M, Du Plessis, SS & Huyser, C 2015, 'Large volume cryoprotectant-free vitrification : an alternative to conventional cryopreservation for human spermatozoa', Andrologia, vol. 47, no. 5, pp. 594-599. |
en_ZA |
dc.identifier.issn |
0303-4569 (print) |
|
dc.identifier.issn |
1439-0272 (online) |
|
dc.identifier.other |
10.1111/and.12307 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/51156 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Wiley |
en_ZA |
dc.rights |
© 2014 Blackwell Verlag GmbH. This is the pre-peer reviewed version of the following article : Large volume cryoprotectant-free vitrification : an alternative to conventional cryopreservation for human spermatozoa, Andrologia, vol. 47, no. 5, pp. 594-599, 2015. doi : 10.1111/and.12307. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1439-0272. |
en_ZA |
dc.subject |
Cryopreservation |
en_ZA |
dc.subject |
DNA integrity |
en_ZA |
dc.subject |
Human spermatozoa |
en_ZA |
dc.subject |
Mitochondrial membrane potential |
en_ZA |
dc.subject |
Vitrification |
en_ZA |
dc.subject |
Deoxyribonucleic acid (DNA) |
en_ZA |
dc.title |
Large volume cryoprotectant-free vitrification : an alternative to conventional cryopreservation for human spermatozoa |
en_ZA |
dc.type |
Postprint Article |
en_ZA |