Large volume cryoprotectant-free vitrification : an alternative to conventional cryopreservation for human spermatozoa
dc.contributor.author | Slabbert, Marisa | |
dc.contributor.author | Du Plessis, S.S. | |
dc.contributor.author | Huyser, Carin | |
dc.date.accessioned | 2015-12-14T05:18:47Z | |
dc.date.issued | 2015-06 | |
dc.description.abstract | Vitrification is a simple and cost-effective method for the storage of human spermatozoa without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. As a result, solidification of living cells without the formation of ice crystals is achieved during cooling. This study aimed to compare cryoprotectant-free vitrification to conventional cryopreservation protocols. Semen samples (n = 35) were collected from patients seeking diagnostic assistance at the Reproductive and Endocrine Unit at Steve Biko Academic Hospital. Samples were processed using a discontinu-ous density-gradient centrifugation method. Washed samples were split into two aliquots and cryopreserved either by means of cryoprotectant-free vitrifica-tion (sucrose + 1% albumin) or conventional slow freezing (TEST-yolk buf-fer). Post-thawing, the sperm motion parameters, mitochondrial membrane potential (Dwm) and DNA fragmentation were compared between the two groups. No significant differences were observed in the sperm motility parame-ters (P > 0.05). Significantly higher percentages of Dwm (11.99% 4.326%versus 6.58% 1.026%; P < 0.001) and lower percentages of DNA fragmenta-tion (2.79% 1.017% versus 3.86% 1.38%; P < 0.01) were observed when comparing cryoprotectant-free vitrification to conventional cryopreservation. Cryoprotectant-free vitrification is a rapid and promising alternative to conventional methods resulting in good-quality spermatozoa post-thaw. | en_ZA |
dc.description.embargo | 2016-06-30 | |
dc.description.librarian | hb2015 | en_ZA |
dc.description.sponsorship | Research Committee of the Faculty of Health Sciences (RESCOM), University of Pretoria, Merck Serono (Pty) Ltd and the National Research Foundation. | en_ZA |
dc.description.uri | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0272 | en_ZA |
dc.identifier.citation | Slabbert, M, Du Plessis, SS & Huyser, C 2015, 'Large volume cryoprotectant-free vitrification : an alternative to conventional cryopreservation for human spermatozoa', Andrologia, vol. 47, no. 5, pp. 594-599. | en_ZA |
dc.identifier.issn | 0303-4569 (print) | |
dc.identifier.issn | 1439-0272 (online) | |
dc.identifier.other | 10.1111/and.12307 | |
dc.identifier.uri | http://hdl.handle.net/2263/51156 | |
dc.language.iso | en | en_ZA |
dc.publisher | Wiley | en_ZA |
dc.rights | © 2014 Blackwell Verlag GmbH. This is the pre-peer reviewed version of the following article : Large volume cryoprotectant-free vitrification : an alternative to conventional cryopreservation for human spermatozoa, Andrologia, vol. 47, no. 5, pp. 594-599, 2015. doi : 10.1111/and.12307. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1439-0272. | en_ZA |
dc.subject | Cryopreservation | en_ZA |
dc.subject | DNA integrity | en_ZA |
dc.subject | Human spermatozoa | en_ZA |
dc.subject | Mitochondrial membrane potential | en_ZA |
dc.subject | Vitrification | en_ZA |
dc.subject | Deoxyribonucleic acid (DNA) | en_ZA |
dc.title | Large volume cryoprotectant-free vitrification : an alternative to conventional cryopreservation for human spermatozoa | en_ZA |
dc.type | Postprint Article | en_ZA |