Comparison of three commercial molecular assays for detection of rifampin and isoniazid resistance among Mycobacterium tuberculosis isolates in a high-HIV-prevalence setting
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Date
Authors
Strydom, Kathy-Anne
Ismail, Farzana
Matabane, M.M.Z.
Onwuegbuna, O.
Omar, Shaheed Vally
Ismail, Nabila
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
Abstract
In a head to head comparison of the MTBDRplus Version 2.0 (Hain Lifescience), Xpert®
MTB/RIF (Cepheid) and the Anyplex™ MTB/NTM (Seegene) assays we demonstrate equal
sensitivity (59/61; 96.7%) and specificity (53/54; 98.1%) for detecting rifampicin resistance
with further analysis of discordances. The Xpert does not detect Isoniazid resistance while
Anyplex showed high false positivity.
Description
Keywords
Rifampicin, Isoniazid, Mycobacterium tuberculosis, High HIV prevalence setting
Sustainable Development Goals
Citation
Strydom, KA, Ismail, F, Matabane, MMZ, Onwuegbuna, O, Omar, SV & Ismail, N 2015, 'Comparison of three commercial molecular assays for detection of rifampin and isoniazid resistance among Mycobacterium tuberculosis isolates in a high-HIV-prevalence setting', Journal of Clinical Microbiology, vol. 53, no. 9, pp. 3032-3034.