Activity-guided isolation and identification of the major antioxidant and anticancer compounds from a commercial Pelargonium sidoides tincture
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Date
Authors
Pereira, Andreia S.P.
Bester, Megan Jean
Soundy, Puffy
Apostolides, Zeno
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Extracts prepared from the roots of Pelargonium sidoides (DC) are commercially available
for the treatment of respiratory related conditions. Recently, a commercial radix mother
tincture of this plant was shown to have both antioxidant and anticancer effects especially
related to the G0/G1 block in the Jurkat E6.1 cell line (unpublished results). Fractions were
prepared by semi-preparative HPLC, and their antioxidant and anticancer activities were
determined. The more hydrophilic fractions isolated namely F6-F12 were all found to have
strong reducing capacities and were able to scavenge peroxyl radicals. In the human lung cell
line, NCI-H460, significant cellular antioxidant effects were observed. Anticancer activity
was evaluated in the NCI-pre-screen panel (NCI-H460, MCF-7 and SF-268) and the Jurkat
E6.1 cell line. Fractions F7, F9 and F12 were found to inhibit the cell growth of these four cell-lines (p < 0.05), especially the Jurkat E6.1 cell line with the sulforhodamine B assay.
Mass spectrometry analysis revealed that these active fractions contained several
polyphenolic compounds such as gallic acid, trihydroxycoumarin, dihydroxycoumarin
sulfates, proanthocyanidins and phenolic glycosides. A phenolic acid glycoside sulfate not
previously shown in Pelargonium sidoides extracts was also isolated. In conclusion, the
antioxidant and/or anticancer activity of the Pelargonium sidoides tincture may be attributed
to the presence of these polyphenolics.
Description
Keywords
Pelargonium sidoides, Activity guided fractionation, Polyphenolic, Coumarin, Anticancer, Antioxidant
Sustainable Development Goals
Citation
Pereira, ASP, Bester, MJ, Soundy, P & Apostolides, Z 2015, 'Activity-guided isolation and identification of the major antioxidant and anticancer compounds from a commercial Pelargonium sidoides tincture', Medicinal Chemistry Research, vol. 24, no. 11, pp. 3838-3852.