During investigations into the volatility of female pheromones of A. walkerae in a Y -shaped olfactometer,
engorged conspecific male ticks responded weakly both to female-contacted filter paper discs and to intact
females enclosed in nylon bags. In tests in which female-contacted filter paper discs were separated from
engorged male ticks by means of a screen, no aggregation was observed. However, replete male ticks showed a
vigorous response to liquefied air and hexane-trapped volatiles collected from intact females.
Guanine showed a potent aggregation attraction for engorged and unfed larvae and 1st and 2nd stage
nymphal ticks as well as for male and female imagines of A. walkerae over a wide concentration range.
It is concluded that at least 2 pheromones, 1 volatile and 1 non-volatile, are operative in the communication
system of this tick species.
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