Abstract:
The present study was carried out to determine the cytotoxic effect of seven plant
extracts and the isolated compounds; syringin and 4-methoxycinnamyl alcohol on
cancerous and non-cancerous cells. The ethanol extract of F. vulgare was found to
exhibit the most significant toxicity with an IC50 value of 19.97 μg/mL on HeLa
cells. Bioassay guided fractionation lead to the isolation of two compounds,
syringin (1) and 4-methoxycinnamyl alcohol (2). Both compounds showed
toxicity against MCF-7, HeLa, and DU145 cancer cell line. The results showed
that compound 2 showed high toxicity against all the cancer cell lines with IC50
values of 14.24, 7.82 and 22.10 μg/mL, respectively. 4-Methoxycinnamyl alcohol
also showed no apoptotic effect in cell cycle analysis after 48 hours at a
concentration of 10 μg/mL. However DNA fragmentation study revealed that,
necrosis took place at a concentration of 10 μg/mL after 48 h exposure.
