Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA

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dc.contributor.author De Flamingh, Alida
dc.contributor.author Sole, Catherine L.
dc.contributor.author Van Aarde, Rudi J.
dc.date.accessioned 2015-02-18T06:14:13Z
dc.date.available 2015-02-18T06:14:13Z
dc.date.issued 2014-09
dc.description.abstract Degradation reduces DNA quality and quantity in faecal samples and leads to low amplification success. We investigated the influence of repeat motif and amplicon length by comparing the amplification success of five dinucleotide, five tetranucleotide, and two compound microsatellite markers for African elephant (Loxodonta africana) faecal DNA samples. We found that both repeat motif and amplicon length influenced amplification success, and suggest the use of simple microsatellite markers containing alleles with small amplicon sizes to maximise amplification success of degraded DNA. en_ZA
dc.description.embargo 2015-09-30 en_ZA
dc.description.librarian hj2015 en_ZA
dc.description.sponsorship The Conservation Ecology Research Unit team, the International Fund for Animal Welfare, the Zambian Wildlife Authority, the Department of Wildlife and National Parks (Botswana), Elephants Without Borders, and the Conservation Foundation (Zambia). en_ZA
dc.description.uri http://link.springer.com/journal/12686 en_ZA
dc.identifier.citation De Flamingh, A, Sole, CL & Van Aarde, RJ 2014, 'Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA', Conservation Genetics Resources, vol. 6, no. 3, pp, 503-505. en_ZA
dc.identifier.issn 1877-7252 (print)
dc.identifier.issn 1877-7260 (online)
dc.identifier.other 10.1007/s12686-014-0160-5
dc.identifier.uri http://hdl.handle.net/2263/43709
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer Science+Business Media Dordrecht 2014. The original publication is available at : http://link.springer.comjournal/12686 en_ZA
dc.subject Repeat motif en_ZA
dc.subject Amplicon length en_ZA
dc.subject Degraded DNA en_ZA
dc.subject Faecal DNA en_ZA
dc.subject African elephant (Loxodonta africana) en_ZA
dc.subject Deoxyribonucleic acid (DNA) en_ZA
dc.title Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA en_ZA
dc.type Postprint Article en_ZA


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