Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA
dc.contributor.author | De Flamingh, Alida | |
dc.contributor.author | Sole, Catherine L. | |
dc.contributor.author | Van Aarde, Rudi J. | |
dc.date.accessioned | 2015-02-18T06:14:13Z | |
dc.date.available | 2015-02-18T06:14:13Z | |
dc.date.issued | 2014-09 | |
dc.description.abstract | Degradation reduces DNA quality and quantity in faecal samples and leads to low amplification success. We investigated the influence of repeat motif and amplicon length by comparing the amplification success of five dinucleotide, five tetranucleotide, and two compound microsatellite markers for African elephant (Loxodonta africana) faecal DNA samples. We found that both repeat motif and amplicon length influenced amplification success, and suggest the use of simple microsatellite markers containing alleles with small amplicon sizes to maximise amplification success of degraded DNA. | en_ZA |
dc.description.embargo | 2015-09-30 | en_ZA |
dc.description.librarian | hj2015 | en_ZA |
dc.description.sponsorship | The Conservation Ecology Research Unit team, the International Fund for Animal Welfare, the Zambian Wildlife Authority, the Department of Wildlife and National Parks (Botswana), Elephants Without Borders, and the Conservation Foundation (Zambia). | en_ZA |
dc.description.uri | http://link.springer.com/journal/12686 | en_ZA |
dc.identifier.citation | De Flamingh, A, Sole, CL & Van Aarde, RJ 2014, 'Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA', Conservation Genetics Resources, vol. 6, no. 3, pp, 503-505. | en_ZA |
dc.identifier.issn | 1877-7252 (print) | |
dc.identifier.issn | 1877-7260 (online) | |
dc.identifier.other | 10.1007/s12686-014-0160-5 | |
dc.identifier.uri | http://hdl.handle.net/2263/43709 | |
dc.language.iso | en | en_ZA |
dc.publisher | Springer | en_ZA |
dc.rights | © Springer Science+Business Media Dordrecht 2014. The original publication is available at : http://link.springer.comjournal/12686 | en_ZA |
dc.subject | Repeat motif | en_ZA |
dc.subject | Amplicon length | en_ZA |
dc.subject | Degraded DNA | en_ZA |
dc.subject | Faecal DNA | en_ZA |
dc.subject | African elephant (Loxodonta africana) | en_ZA |
dc.subject | Deoxyribonucleic acid (DNA) | en_ZA |
dc.title | Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA | en_ZA |
dc.type | Postprint Article | en_ZA |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- DeFlamingh_Microsatellite_2014.pdf
- Size:
- 194.89 KB
- Format:
- Adobe Portable Document Format
- Description:
- Postprint Article
License bundle
1 - 1 of 1
Loading...
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: