Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA

dc.contributor.authorDe Flamingh, Alida
dc.contributor.authorSole, Catherine L.
dc.contributor.authorVan Aarde, Rudi J.
dc.date.accessioned2015-02-18T06:14:13Z
dc.date.available2015-02-18T06:14:13Z
dc.date.issued2014-09
dc.description.abstractDegradation reduces DNA quality and quantity in faecal samples and leads to low amplification success. We investigated the influence of repeat motif and amplicon length by comparing the amplification success of five dinucleotide, five tetranucleotide, and two compound microsatellite markers for African elephant (Loxodonta africana) faecal DNA samples. We found that both repeat motif and amplicon length influenced amplification success, and suggest the use of simple microsatellite markers containing alleles with small amplicon sizes to maximise amplification success of degraded DNA.en_ZA
dc.description.embargo2015-09-30en_ZA
dc.description.librarianhj2015en_ZA
dc.description.sponsorshipThe Conservation Ecology Research Unit team, the International Fund for Animal Welfare, the Zambian Wildlife Authority, the Department of Wildlife and National Parks (Botswana), Elephants Without Borders, and the Conservation Foundation (Zambia).en_ZA
dc.description.urihttp://link.springer.com/journal/12686en_ZA
dc.identifier.citationDe Flamingh, A, Sole, CL & Van Aarde, RJ 2014, 'Microsatellite repeat motif and amplicon length affect amplification success of degraded faecal DNA', Conservation Genetics Resources, vol. 6, no. 3, pp, 503-505.en_ZA
dc.identifier.issn1877-7252 (print)
dc.identifier.issn1877-7260 (online)
dc.identifier.other10.1007/s12686-014-0160-5
dc.identifier.urihttp://hdl.handle.net/2263/43709
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer Science+Business Media Dordrecht 2014. The original publication is available at : http://link.springer.comjournal/12686en_ZA
dc.subjectRepeat motifen_ZA
dc.subjectAmplicon lengthen_ZA
dc.subjectDegraded DNAen_ZA
dc.subjectFaecal DNAen_ZA
dc.subjectAfrican elephant (Loxodonta africana)en_ZA
dc.subjectDeoxyribonucleic acid (DNA)en_ZA
dc.titleMicrosatellite repeat motif and amplicon length affect amplification success of degraded faecal DNAen_ZA
dc.typePostprint Articleen_ZA

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