dc.contributor.author |
Bello, Umar M.
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dc.contributor.author |
Madekurozwa, Mary-Catherine
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dc.contributor.author |
Groenewald, Hermanus B.
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dc.contributor.author |
Aire, Tom A.
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dc.contributor.author |
Arukwe, Augustine
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dc.date.accessioned |
2015-02-18T05:44:15Z |
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dc.date.available |
2015-02-18T05:44:15Z |
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dc.date.issued |
2014-11 |
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dc.description.abstract |
In the present study, we have investigated the effects of 30-day dietary (pre-pubertal) exposure to different doses (0 (control), 1, 10, 50, 200 and 400 mg/kg bodyweight/day) of di(n-butyl) phthalate (DBP) on Leydig cells of adult male Japanese quails by quantifying the transcript levels for P450 side-chain cleavage (p450scc), P450c17 (CYP17), and 3β- and 17β-hydroxysteroid dehydrogenase (hsd) using quantitative (real-time) polymerase chain reaction (qRT-PCR). In addition, the plasma testosterone levels were analysed using radioimmunoassay (RIA) and testis was examined for evidence of gross pathology and histopathology. Our data showed that pre-pubertal exposure to DBP produced alterations in testicular architecture as evident by poorly developed or mis-shaped testis, and altered spermatogenesis due to tubular degeneration and atrophy of seminiferous tubules especially in the high DBP dose (200 and 400 mg/kg) treated groups. In addition, DBP altered several key enzymes involved in testicular steroidogenesis pathways in an apparent dose-dependent manner. For example, biphasic effects of DBP were observed for P450scc and 3β-hsd mRNA, that were generally increasing at low dose 10 mg/kg, and thereafter, an apparent dose-dependent decrease between 50 and 400 mg/kg. The steroidogenic acute regulatory (StAR) protein was at the lowest detectable limits and therefore not quantifiable. These effects did not parallel the non-significant changes observed for plasma testosterone levels. The present data is consistent with previous reports showing that DBP modulates Leydig cell steroidogenesis in several species, with a potential negative effect on reproduction in those avian species that are vulnerable to endocrine disrupting chemicals. |
en_ZA |
dc.description.librarian |
hj2015 |
en_ZA |
dc.description.sponsorship |
The Norwegian Research Council (NRC) (Grant number 1964442/S40) to AA. |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/cbpc |
en_ZA |
dc.identifier.citation |
Bello, UM, Madekurozwa, MC, Groenewald, HB, Aire, TA & Arukwe, A 2014, 'The effects on steroidogenesis and histopathology of adult male Japanese quails (Coturnix coturnix japonica) testis following pre-pubertal exposure to di(n-butyl) phthalate (DBP)', Comparative Biochemistry and Physiology Part C : Toxicology and Pharmacology, vol. 166, pp. 24-33. |
en_ZA |
dc.identifier.issn |
1532-0456 (print) |
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dc.identifier.issn |
1878-1659 (online) |
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dc.identifier.other |
10.1016/j.cbpc.2014.06.005 |
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dc.identifier.other |
O-7064-2014 |
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dc.identifier.other |
6603322006 |
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dc.identifier.uri |
http://hdl.handle.net/2263/43708 |
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dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2014 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Comparative Biochemistry and Physiology Part C. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Comparative Biochemistry and Physiology Part C, vol. 166, pp.24-33, 2014. doi : 10.1016/j.cbpc.2014.06.005. |
en_ZA |
dc.subject |
Di-(n-butyl) phthalate |
en_ZA |
dc.subject |
Leydig cell |
en_ZA |
dc.subject |
Steroidogenesis |
en_ZA |
dc.subject |
Male Japanese quails |
en_ZA |
dc.subject |
Endocrine disruption |
en_ZA |
dc.title |
The effects on steroidogenesis and histopathology of adult male Japanese quails (Coturnix coturnix japonica) testis following pre-pubertal exposure to di(n-butyl) phthalate (DBP) |
en_ZA |
dc.type |
Postprint Article |
en_ZA |