Volatiles emitted by male Amblyomma hebraeum while feeding on a rabbit were quantitatively sampled by
the dynamic solvent effect and analysed by capillary gas-liquid chromatography and mass spectrometry.
Changes in emissions of 2-methyl propanoic acid, benzaldehyde and 2-nitrophenol paralleled reported increases
in the attractiveness of males to conspecific ticks after 4-5 days of feeding.
These compounds are therefore candidates for the bioassay of pheromonal activity.