An investigation into alternative methods for the diagnosis of dourine

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Authors

Williamson, Catherine C.
Mattheus, A.
Schiele, G.J.

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Published by the Government Printer, Pretoria.

Abstract

The complement fixation test (CFT), indirect fluorescent antibody test (IFAT), card agglutination test for trypanosomiasis (CATT) and enzyme-linked immunosorbent assay (ELISA) were compared in their application to the serological diagnosis of Trypanosoma equiperdum infection in 43 horses. The CFT remains a reliable test for dourine, especially in countries where other members of the subgenus Trypanozoon do not occur. The IFAT is a Good 'back-up' test, but, requiring skilled operators it has the disadvantage of making it labour intensive, and interpretation of results subjective. This makes it more suited to small numbers of samples. The ELISA is suitable for large numbers of samples and could readily be used in routine diagnostic procedures. The CATT could be of value in field situations, although it does not appear to be as sensitive as the CFT. Its possible application under these conditions should be further investigated.

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The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.

Keywords

Veterinary medicine, Dourine

Sustainable Development Goals

Citation

Williamson, CC, Stoltsz, WH, Mattheus, A & Schiele, GJ 1988, 'An investigation into alternative methods for the diagnosis of dourine', Onderstepoort Journal of Veterinary Research, vol. 55, no. 2, pp. 117-119.