Hybrids between Rhipicephalus appendiculatus and R. zambeziensis were reared and glucose-phosphate-isomerase isoenzymes were resolved by agarose electrophoresis. By phenotyping hybrids in F₁ and F₂ generations autosomal transmission of 2 GPI genes was demonstrated. Identification of a hybrid phenotype provides a method for identifying hybrids in field collections.
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