Antigenic characterisation of lyssaviruses in South Africa

Show simple item record Ngoepe, Chuene Ernest Fehlner-Gardiner, Christine Wandeler, A.I. (Alexander I.) Sabeta, Claude Taurai 2014-09-30T12:53:00Z 2014-09-30T12:53:00Z 2014-09-11
dc.description.abstract There are at least six Lyssavirus species that have been isolated in Africa, which include classical rabies virus, Lagos bat virus, Mokola virus, Duvenhage virus, Shimoni bat virus and Ikoma lyssavirus. In this retrospective study, an analysis of the antigenic reactivity patterns of lyssaviruses in South Africa against a panel of 15 anti-nucleoprotein monoclonal antibodies was undertaken. A total of 624 brain specimens, collected between 2005 and 2009, confirmed as containing lyssavirus antigen by direct fluorescent antibody test, were subjected to antigenic differentiation. The lyssaviruses were differentiated into two species, namely rabies virus (99.5%) and Mokola virus (0.5%). Furthermore, rabies virus was further delineated into two common rabies biotypes in South Africa: canid and mongoose. Initially, it was found that the canid rabies biotype had two reactivity patterns; differential staining was observed with just one monoclonal antibody. This difference was likely to have been an artefact related to sample quality, as passage in cell culture restored staining. Mongoose rabies viruses were more heterogeneous, with seven antigenic reactivity patterns detected. Although Mokola viruses were identified in this study, prevalence and reservoir host species are yet to be established. These data demonstrate the usefulness of monoclonal antibody typing panels in lyssavirus surveillance with reference to emergence of new species or spread of rabies biotypes to new geographic zones. en_US
dc.description.librarian am2014 en_US
dc.description.sponsorship This work was partly funded by the Rabies Diagnostic Project, Onderstepoort Veterinary Research Institute (OVI 15/4/P001) and the European Virus Archive (EVA) (04/17/C215). en_US
dc.description.uri en_US
dc.identifier.citation Ngoepe, E., Fehlner-Gardiner, C., Wandeler, A. & Sabeta, C., 2014, ‘Antigenic characterisation of lyssaviruses in South Africa’, Onderstepoort Journal of Veterinary Research 81(1), Art. #711, 9 pages. 10.4102/ojvr.v81i1.711. en_US
dc.identifier.issn 0030-2465 (print)
dc.identifier.issn 2219-0635 (online)
dc.identifier.other 10.4102/ojvr.v81i1.711
dc.language.iso en en_US
dc.publisher AOSIS Open Journals en_US
dc.relation.requires Adobe Acrobat Reader en
dc.rights © 2014. The Authors. Licensee: AOSIS OpenJournals. This work is licensed under the Creative Commons Attribution License. en_US
dc.subject Lyssaviruses en_US
dc.subject South Africa en_US
dc.subject Rabies virus en_US
dc.subject Lagos bat virus en_US
dc.subject Mokola virus en_US
dc.subject Duvenhage virus en_US
dc.subject Shimoni bat virus en_US
dc.subject Ikoma lyssavirus en_US
dc.title Antigenic characterisation of lyssaviruses in South Africa en_US
dc.type Article en_US

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