Antigenic characterisation of lyssaviruses in South Africa

dc.contributor.authorNgoepe, Chuene Ernest
dc.contributor.authorFehlner-Gardiner, Christine
dc.contributor.authorWandeler, A.I. (Alexander I.)
dc.contributor.authorSabeta, Claude Taurai
dc.date.accessioned2014-09-30T12:53:00Z
dc.date.available2014-09-30T12:53:00Z
dc.date.issued2014-09-11
dc.description.abstractThere are at least six Lyssavirus species that have been isolated in Africa, which include classical rabies virus, Lagos bat virus, Mokola virus, Duvenhage virus, Shimoni bat virus and Ikoma lyssavirus. In this retrospective study, an analysis of the antigenic reactivity patterns of lyssaviruses in South Africa against a panel of 15 anti-nucleoprotein monoclonal antibodies was undertaken. A total of 624 brain specimens, collected between 2005 and 2009, confirmed as containing lyssavirus antigen by direct fluorescent antibody test, were subjected to antigenic differentiation. The lyssaviruses were differentiated into two species, namely rabies virus (99.5%) and Mokola virus (0.5%). Furthermore, rabies virus was further delineated into two common rabies biotypes in South Africa: canid and mongoose. Initially, it was found that the canid rabies biotype had two reactivity patterns; differential staining was observed with just one monoclonal antibody. This difference was likely to have been an artefact related to sample quality, as passage in cell culture restored staining. Mongoose rabies viruses were more heterogeneous, with seven antigenic reactivity patterns detected. Although Mokola viruses were identified in this study, prevalence and reservoir host species are yet to be established. These data demonstrate the usefulness of monoclonal antibody typing panels in lyssavirus surveillance with reference to emergence of new species or spread of rabies biotypes to new geographic zones.en_US
dc.description.librarianam2014en_US
dc.description.sponsorshipThis work was partly funded by the Rabies Diagnostic Project, Onderstepoort Veterinary Research Institute (OVI 15/4/P001) and the European Virus Archive (EVA) (04/17/C215).en_US
dc.description.urihttp://www.ojvr.orgen_US
dc.identifier.citationNgoepe, E., Fehlner-Gardiner, C., Wandeler, A. & Sabeta, C., 2014, ‘Antigenic characterisation of lyssaviruses in South Africa’, Onderstepoort Journal of Veterinary Research 81(1), Art. #711, 9 pages. http://dx.DOI.org/ 10.4102/ojvr.v81i1.711.en_US
dc.identifier.issn0030-2465 (print)
dc.identifier.issn2219-0635 (online)
dc.identifier.other10.4102/ojvr.v81i1.711
dc.identifier.urihttp://hdl.handle.net/2263/42162
dc.language.isoenen_US
dc.publisherAOSIS Open Journalsen_US
dc.relation.requiresAdobe Acrobat Readeren
dc.rights© 2014. The Authors. Licensee: AOSIS OpenJournals. This work is licensed under the Creative Commons Attribution License.en_US
dc.subjectLyssavirusesen_US
dc.subjectSouth Africaen_US
dc.subjectRabies virusen_US
dc.subjectLagos bat virusen_US
dc.subjectMokola virusen_US
dc.subjectDuvenhage virusen_US
dc.subjectShimoni bat virusen_US
dc.subjectIkoma lyssavirusen_US
dc.titleAntigenic characterisation of lyssaviruses in South Africaen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Ngoepe_Antigenic_2014.pdf
Size:
798.34 KB
Format:
Adobe Portable Document Format
Description:
Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: