Ultrastructural comparison of the morphology of three different platelet and fibrin fiber preparations
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Date
Authors
Pretorius, Etheresia
Briedenhann, Sharon
Marx, Jorika
Smit, Eureka
Van der Merwe, Christiaan F.
Pieters, Marlien
Franz, Carl
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
The aim of the current study was to investigate the ultrastructural morphology of three different sources of fibrin networks and platelets, namely, lypholized human platelet-rich plasma (LPRP), freshly prepared human platelet-rich plasma (FPRP), and human platelet concentrate (HPC). The ultrastructural morphology of the three different fibrin networks was studied using the scanning electron microscope (SEM). Turbidity curves were drawn at 405 nm at room temperature and fibrinogen concentrations were measured. Scanning electron micrographs showed that all clots produced thick major fibrin fibers as well as a well-defined fine fibrin network, which appeared to be a superimposed process that occurred after the major fibrin network was established. These features were decidedly more pronounced in the HPC specimens. Turbidity curves of the three types of plasma showed differences in LPRP and FPRP. Fibrinogen concentrations of all three preparations were in the normal ranges. Because of the great similarity between LPRP, HPC, and FPRP, we suggest that LPRP could be used successfully to study morphological changes in fibrin fibers and platelets, which may occur after exposure to certain therapeutic agents. However, functionality studies such as turbidity curves should concurrently be included. We therefore conclude that from a basic science point of view, LPRP is a valuable research tool and that such results may add information that could be valuable for clinical application.
Description
Keywords
Lyophilized human platelet-rich plasma, Fibrin network, Scanning electron microscopy, Human platelet concentrate, Platelet-rich plasma
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Citation
Pretorius, E, Briedenhann, S, Marx, J, Smit, E, Van der Merwe, C, Pieters, M & Franz, C 2007, 'Ultrastructural comparison of the morphology of three different platelet and fibrin fiber preparations', Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, vol. 290, no. 2, pp. 188-198. [http://eu.wiley.com/WileyCDA/WileyTitle/productCd-AR.html]