Browsing South African National Veterinary Repository by Subject "Detection"

Browsing South African National Veterinary Repository by Subject "Detection"

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  • Detection of bluetongue virus and African horsesickness virus in co-infected cell cultures with NS1 gene probes 
    Venter, Estelle Hildegard; Huismans, H. (Henk), 1942-; Van Dijk, A.A.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)
    The serogroup specificity of the bluetongue virus (BTV) NS1 and VP3 gene probes was confirmed by means of northern blot hybridization. Under high-stringency conditions both probes hybridized to 22 BTV serotypes (18 South ...
  • Detection of bovine-virus-diarrhoea-virus antibodies in cattle with an enzyme-linked immunosorbent assay 
    Muvavarirwa, P.; Mudenge, D.; Moyo, Doreen Zandile; Javangwe, S.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)
    The serum-neutralization (SN) and the indirect-immunofluorescence (IIF) assays have invariably been used for detecting antibodies against bovine virus diarrhoea virus (BVDV) in cattle sera. An enzyme-linked immunosorbent ...
  • Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR 
    Wang, Zheng-Wei; Vreede, F.T.; Mitchell, J.O.; Viljoen, G.J.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2001)
    A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein ...