Detection of bovine-virus-diarrhoea-virus antibodies in cattle with an enzyme-linked immunosorbent assay
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Date
Authors
Muvavarirwa, P.
Mudenge, D.
Moyo, Doreen Zandile
Javangwe, S.
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Agricultural Research Council, Onderstepoort Veterinary Institute
Abstract
The serum-neutralization (SN) and the indirect-immunofluorescence (IIF) assays have invariably been
used for detecting antibodies against bovine virus diarrhoea virus (BVDV) in cattle sera. An enzyme-linked immunosorbent assay (ELISA) was applied which has a sensitivity comparable with the SN and IIF in detecting antibody to BVDV. A total of 472 bovine sera were assayed and a high prevalence of
79,2% was recorded. Positive correlations between the ELISA and the SN were found when certain
sera were assayed, implying that the former test could then be used for routine diagnosis of BVDV.
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Keywords
Veterinary medicine, Antibodies, Bovine viral diarrhoea virus (BVDV), BVDV, Cattle, Detection, Enzyme linked, Immunosorbent assay
Sustainable Development Goals
Citation
Muvavarirwa, P, Mudenge, D, Moyo, D & Javangwe, S 1995, 'Detection of bovine-virus-diarrhoea-virus antibodies in cattle with an enzyme-linked immunosorbent assay’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 4, pp. 241-244.