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Browsing South African National Veterinary Repository by Author "Ambrosio, R.E."
Now showing items 1-6 of 6
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Ambrosio, R.E.; Potgieter, F.T.; Nel, N.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1986)A cellulose column procedure is described which removes white cells from bovine blood infected with Babesia and Anaplasma. The efficiency of this method was confirmed by the absence of white blood cell DNA in lysates from ...
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Ambrosio, R.E.; Du Plessis, J.L.; Bezuidenhout, J.D.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)Genomic libraries of the Welgevonden and Kwanyanga isolates of Cowdria ruminantium have been constructed in an expression vector. These libraries contain approximately 4 x 10⁵ and 3 x 10⁵ recombinants respectively.
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Visser, Elizabeth S.; Ambrosio, R.E.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)Anaplasmosis can be diagnosed either by immunological techniques or by direct microscopic examination of blood smears. Both methods are time-consuming and labour intensive. The use of DNA probes in an hybridization assay ...
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Ambrosio, R.E.; Potgieter, F.T.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)The Tm value of DNA from Anaplasma centrale and Anaplasma marginale was found to be 87,1 °C and 89,3 °C, respectively. The G + C content, calculated from the Tm, was 45,1% for A. centrale and 48,5 % for A. marginale. ...
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Ambrosio, R.E.; Visser, Elizabeth S.; Koekhoven, Yolanda; Kocan, Katherine M.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1988)DNA from the Washington, South-Idaho, Virginia and Florida isolates of Anaplasma marginale was hybridized to probes specific for Anaplasma centrale and A. marginale. The A. centrale probes AC-2 and AC-4 hybridized to ...
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Wilkins, S.C.; Ambrosio, R.E.; Verwoerd, Daniel Wynand (Published by the Government Printer, Pretoria., 1989)Screening of a genomic library of Cowdria ruminantium has yielded twelve clones hybridizing to Cowdria DNA. These clones should be suitable for development into DNA probes specific for this organism.
Now showing items 1-6 of 6