South African papilionoid legumes are nodulated by diverse Burkholderia with unique nodulation and nitrogen-fixation Loci

Show simple item record Beukes, Chrizelle Winsie Venter, S.N. (Stephanus Nicolaas) Law, Ian J. Phalane, Francina Lebogang Steenkamp, Emma Theodora 2014-04-01T10:14:30Z 2014-04-01T10:14:30Z 2013-07-11
dc.description.abstract The root-nodule bacteria of legumes endemic to the Cape Floristic Region are largely understudied, even though recent reports suggest the occurrence of nodulating Burkholderia species unique to the region. In this study, we considered the diversity and evolution of nodulating Burkholderia associated with the endemic papilionoid tribes Hypocalypteae and Podalyrieae. We identified distinct groups from verified rhizobial isolates by phylogenetic analyses of the 16S rRNA and recA housekeeping gene regions. In order to gain insight into the evolution of the nodulation and diazotrophy of these rhizobia we analysed the genes encoding NifH and NodA. The majority of these 69 isolates appeared to be unique, potentially representing novel species. Evidence of horizontal gene transfer determining the symbiotic ability of these Cape Floristic Region isolates indicate evolutionary origins distinct from those of nodulating Burkholderia from elsewhere in the world. Overall, our findings suggest that Burkholderia species associated with fynbos legumes are highly diverse and their symbiotic abilities have unique ancestries. It is therefore possible that the evolution of these bacteria is closely linked to the diversification and establishment of legumes characteristic of the Cape Floristic Region. en_US
dc.description.librarian am2014 en_US
dc.description.sponsorship The National Research Foundation (NRF) (, the University of Pretoria ( and the Centre of Excellence in Tree Health Biotechnology (CTHB) of the Department of Science and Technology (DST) ( en_US
dc.description.uri en_US
dc.identifier.citation Beukes CW, Venter SN, Law IJ, Phalane FL, Steenkamp ET (2013) South African Papilionoid Legumes Are Nodulated by Diverse Burkholderia with Unique Nodulation and Nitrogen-Fixation Loci. PLoS ONE 8(7): e68406. DOI: 10.1371/journal.pone.0068406 en_US
dc.identifier.issn 1935-2727
dc.identifier.other 10.1371/journal.pone.0068406
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.rights © 2013 Beukes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License en_US
dc.subject Papilionoid legumes en_US
dc.subject Bacteria en_US
dc.subject Burkholderia en_US
dc.subject Nitrogen-fixation loci en_US
dc.subject Cape floristic region en_US
dc.title South African papilionoid legumes are nodulated by diverse Burkholderia with unique nodulation and nitrogen-fixation Loci en_US
dc.type Article en_US

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