South African papilionoid legumes are nodulated by diverse Burkholderia with unique nodulation and nitrogen-fixation Loci

dc.contributor.authorBeukes, Chrizelle Winsie
dc.contributor.authorVenter, S.N. (Stephanus Nicolaas)
dc.contributor.authorLaw, Ian J.
dc.contributor.authorPhalane, Francina Lebogang
dc.contributor.authorSteenkamp, Emma Theodora
dc.contributor.emailchrizelle.beukes@fabi.up.ac.zaen_US
dc.date.accessioned2014-04-01T10:14:30Z
dc.date.available2014-04-01T10:14:30Z
dc.date.issued2013-07-11
dc.description.abstractThe root-nodule bacteria of legumes endemic to the Cape Floristic Region are largely understudied, even though recent reports suggest the occurrence of nodulating Burkholderia species unique to the region. In this study, we considered the diversity and evolution of nodulating Burkholderia associated with the endemic papilionoid tribes Hypocalypteae and Podalyrieae. We identified distinct groups from verified rhizobial isolates by phylogenetic analyses of the 16S rRNA and recA housekeeping gene regions. In order to gain insight into the evolution of the nodulation and diazotrophy of these rhizobia we analysed the genes encoding NifH and NodA. The majority of these 69 isolates appeared to be unique, potentially representing novel species. Evidence of horizontal gene transfer determining the symbiotic ability of these Cape Floristic Region isolates indicate evolutionary origins distinct from those of nodulating Burkholderia from elsewhere in the world. Overall, our findings suggest that Burkholderia species associated with fynbos legumes are highly diverse and their symbiotic abilities have unique ancestries. It is therefore possible that the evolution of these bacteria is closely linked to the diversification and establishment of legumes characteristic of the Cape Floristic Region.en_US
dc.description.librarianam2014en_US
dc.description.sponsorshipThe National Research Foundation (NRF) (www.nrf.ac.za), the University of Pretoria (web.up.ac.za) and the Centre of Excellence in Tree Health Biotechnology (CTHB) of the Department of Science and Technology (DST) (www.dst.gov.za).en_US
dc.description.uriwww.plosone.orgen_US
dc.identifier.citationBeukes CW, Venter SN, Law IJ, Phalane FL, Steenkamp ET (2013) South African Papilionoid Legumes Are Nodulated by Diverse Burkholderia with Unique Nodulation and Nitrogen-Fixation Loci. PLoS ONE 8(7): e68406. DOI: 10.1371/journal.pone.0068406en_US
dc.identifier.issn1935-2727
dc.identifier.other10.1371/journal.pone.0068406
dc.identifier.urihttp://hdl.handle.net/2263/37342
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.rights© 2013 Beukes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution Licenseen_US
dc.subjectPapilionoid legumesen_US
dc.subjectBacteriaen_US
dc.subjectBurkholderiaen_US
dc.subjectNitrogen-fixation locien_US
dc.subjectCape floristic regionen_US
dc.titleSouth African papilionoid legumes are nodulated by diverse Burkholderia with unique nodulation and nitrogen-fixation Locien_US
dc.typeArticleen_US

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