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dc.contributor.author | Anyango, Joseph Ochieng | |
dc.contributor.author | Taylor, J.R.N. (John Reginald Nuttall) | |
dc.contributor.author | Taylor, Janet | |
dc.date.accessioned | 2014-03-31T07:15:27Z | |
dc.date.available | 2014-12-01T00:20:06Z | |
dc.date.issued | 2013-11 | |
dc.description.abstract | The possible importance of the cysteine-rich γ-prolamin in kafirin and zein functionality has been neglected. The role of γ-kafirin in organized microstructures was investigated in microparticles. Residual kafirin (total kafirin minus γ-kafirin) “microparticles” were non-discrete (amorphous mass of material), as viewed by electron microscopy and atomic force microscopy. Adding 15% γ-kafirin to residual kafirin resulted in the formation of a mixture of non-discrete material and nanosize discrete spherical structures. Adding 30% γ-kafirin to the residual kafirin resulted in discrete spherical nanosize particles. Fourier transform infrared spectroscopy indicated that γ-kafirin had a mixture of random-coil and β-sheet conformations, in contrast to total kafirin, which is mainly α-helical conformation. γ-Kafirin also had a very high glass transition temperature (Tg) (≈270 °C). The conformation and high Tg of γ-kafirin probably confer structural stability to kafirin microstructures. Because of its ability to form disulfide cross-links, γ-kafirin appears to be essential to form and stabilize organized microstructures. | en_US |
dc.description.librarian | hb2014 | en_US |
dc.description.sponsorship | Joseph Anyango is grateful for the provision of a University of Pretoria Postdoctoral Fellowship. | en_US |
dc.description.uri | http://pubs.acs.org/journal/jafcau | en_US |
dc.identifier.citation | Anyango, JO, Taylor, JRN & Taylor, J 2013, 'Role of γ-kafirin in the formation and organization of kafirin microstructures', Journal of Agricultural and Food Chemistry, vol. 61, no. 45, pp. 10757-10765. | en_US |
dc.identifier.issn | 0021-8561 (print) | |
dc.identifier.issn | 1520-5118 (online) | |
dc.identifier.other | 10.1021/jf403571e | |
dc.identifier.uri | http://hdl.handle.net/2263/37211 | |
dc.language.iso | en | en_US |
dc.publisher | American Chemical Society | en_US |
dc.rights | © 2013 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, vol. 61, no. 45, pp. 10757-10765, 2013. doi : 10.1021/jf403571e | en_US |
dc.subject | Kafirin microparticles | en_US |
dc.subject | Disulfide cross-linking | en_US |
dc.subject | Glass transition temperature | en_US |
dc.subject | Secondary structure | en_US |
dc.subject | Y-kafirin | en_US |
dc.title | Role of γ-kafirin in the formation and organization of kafirin microstructures | en_US |
dc.type | Postprint Article | en_US |