dc.contributor.author |
Anyango, Joseph Ochieng
|
|
dc.contributor.author |
Taylor, J.R.N. (John Reginald Nuttall)
|
|
dc.contributor.author |
Taylor, Janet
|
|
dc.date.accessioned |
2014-03-31T07:15:27Z |
|
dc.date.available |
2014-12-01T00:20:06Z |
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dc.date.issued |
2013-11 |
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dc.description.abstract |
The possible importance of the cysteine-rich γ-prolamin in kafirin and zein functionality has been neglected. The role of γ-kafirin in organized microstructures was investigated in microparticles. Residual kafirin (total kafirin minus γ-kafirin) “microparticles” were non-discrete (amorphous mass of material), as viewed by electron microscopy and atomic force microscopy. Adding 15% γ-kafirin to residual kafirin resulted in the formation of a mixture of non-discrete material and nanosize discrete spherical structures. Adding 30% γ-kafirin to the residual kafirin resulted in discrete spherical nanosize particles. Fourier transform infrared spectroscopy indicated that γ-kafirin had a mixture of random-coil and β-sheet conformations, in contrast to total kafirin, which is mainly α-helical conformation. γ-Kafirin also had a very high glass transition temperature (Tg) (≈270 °C). The conformation and high Tg of γ-kafirin probably confer structural stability to kafirin microstructures. Because of its ability to form disulfide cross-links, γ-kafirin appears to be essential to form and stabilize organized microstructures. |
en_US |
dc.description.librarian |
hb2014 |
en_US |
dc.description.sponsorship |
Joseph Anyango is grateful for the provision of a University of Pretoria Postdoctoral Fellowship. |
en_US |
dc.description.uri |
http://pubs.acs.org/journal/jafcau |
en_US |
dc.identifier.citation |
Anyango, JO, Taylor, JRN & Taylor, J 2013, 'Role of γ-kafirin in the formation and organization of kafirin microstructures', Journal of Agricultural and Food Chemistry, vol. 61, no. 45, pp. 10757-10765. |
en_US |
dc.identifier.issn |
0021-8561 (print) |
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dc.identifier.issn |
1520-5118 (online) |
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dc.identifier.other |
10.1021/jf403571e |
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dc.identifier.uri |
http://hdl.handle.net/2263/37211 |
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dc.language.iso |
en |
en_US |
dc.publisher |
American Chemical Society |
en_US |
dc.rights |
© 2013 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, vol. 61, no. 45, pp. 10757-10765, 2013. doi : 10.1021/jf403571e |
en_US |
dc.subject |
Kafirin microparticles |
en_US |
dc.subject |
Disulfide cross-linking |
en_US |
dc.subject |
Glass transition temperature |
en_US |
dc.subject |
Secondary structure |
en_US |
dc.subject |
Y-kafirin |
en_US |
dc.title |
Role of γ-kafirin in the formation and organization of kafirin microstructures |
en_US |
dc.type |
Postprint Article |
en_US |