Role of γ-kafirin in the formation and organization of kafirin microstructures

dc.contributor.authorAnyango, Joseph Ochieng
dc.contributor.authorTaylor, J.R.N. (John Reginald Nuttall)
dc.contributor.authorTaylor, Janet
dc.contributor.emailjanet.taylor@up.ac.zaen_US
dc.date.accessioned2014-03-31T07:15:27Z
dc.date.available2014-12-01T00:20:06Z
dc.date.issued2013-11
dc.description.abstractThe possible importance of the cysteine-rich γ-prolamin in kafirin and zein functionality has been neglected. The role of γ-kafirin in organized microstructures was investigated in microparticles. Residual kafirin (total kafirin minus γ-kafirin) “microparticles” were non-discrete (amorphous mass of material), as viewed by electron microscopy and atomic force microscopy. Adding 15% γ-kafirin to residual kafirin resulted in the formation of a mixture of non-discrete material and nanosize discrete spherical structures. Adding 30% γ-kafirin to the residual kafirin resulted in discrete spherical nanosize particles. Fourier transform infrared spectroscopy indicated that γ-kafirin had a mixture of random-coil and β-sheet conformations, in contrast to total kafirin, which is mainly α-helical conformation. γ-Kafirin also had a very high glass transition temperature (Tg) (≈270 °C). The conformation and high Tg of γ-kafirin probably confer structural stability to kafirin microstructures. Because of its ability to form disulfide cross-links, γ-kafirin appears to be essential to form and stabilize organized microstructures.en_US
dc.description.librarianhb2014en_US
dc.description.sponsorshipJoseph Anyango is grateful for the provision of a University of Pretoria Postdoctoral Fellowship.en_US
dc.description.urihttp://pubs.acs.org/journal/jafcauen_US
dc.identifier.citationAnyango, JO, Taylor, JRN & Taylor, J 2013, 'Role of γ-kafirin in the formation and organization of kafirin microstructures', Journal of Agricultural and Food Chemistry, vol. 61, no. 45, pp. 10757-10765.en_US
dc.identifier.issn0021-8561 (print)
dc.identifier.issn1520-5118 (online)
dc.identifier.other10.1021/jf403571e
dc.identifier.urihttp://hdl.handle.net/2263/37211
dc.language.isoenen_US
dc.publisherAmerican Chemical Societyen_US
dc.rights© 2013 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, vol. 61, no. 45, pp. 10757-10765, 2013. doi : 10.1021/jf403571een_US
dc.subjectKafirin microparticlesen_US
dc.subjectDisulfide cross-linkingen_US
dc.subjectGlass transition temperatureen_US
dc.subjectSecondary structureen_US
dc.subjectY-kafirinen_US
dc.titleRole of γ-kafirin in the formation and organization of kafirin microstructuresen_US
dc.typePostprint Articleen_US

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