Isolation of the toxin responsible for gousiekte, a plant-induced cardiomyopathy of ruminants in southern Africa

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dc.contributor.author Fourie, Nicole
dc.contributor.author Erasmus, G.L.
dc.contributor.author Schultz, R.A. (Rowena Anitra)
dc.contributor.editor Verwoerd, Daniel Wynand
dc.contributor.upauthor Prozesky, Leon
dc.date.accessioned 2013-09-12T08:41:28Z
dc.date.available 2013-09-12T08:41:28Z
dc.date.created 2013
dc.date.issued 1995
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en
dc.description.abstract Gousiekte was induced in sheep and goats by the oral and intravenous (i/v) administration of methanolic and ethanolic precipitates of aqueous extracts of Pavetta harborii. Further purification of the toxic principle was carried out by dialysis and ion-exchange chromatography. The toxin was eluted from the cation-exchange resin with 1 mol/l of ammonium acetate and finally purified on Sephadex G-10 and silica gel. Spots on thin-layer chromatographic plates (TLC) were visualized with ninhydrin. Gousiekte was induced with the pure toxin after i/v administration in goats. The yield of pure toxin was about 10 mg/kg of dried leaves. A compound, indistinguishable from the above on TLC, was also isolated from the gousiekte-inducing plants Pavetta schumanniana, Fadogia homblei and Pachystigma pygmaeum. en
dc.description.librarian mn2013
dc.identifier.citation Fourie, N, Erasmus, GL, Schultz, RA & Prozesky, L 1995, 'Isolation of the toxin responsible for gousiekte, a plant-induced cardiomyopathy of ruminants in southern Africa’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 2, pp. 77-87. en
dc.identifier.issn 0330-2465
dc.identifier.uri http://hdl.handle.net/2263/31699
dc.language.iso en en
dc.publisher Published by the Agricultural Research Council, Onderstepoort Veterinary Institute en
dc.rights © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). en
dc.subject Veterinary medicine en
dc.subject Gousiekte en
dc.subject Cardiomyopathy en
dc.subject Cardiac failure en
dc.subject Plant poisoning en
dc.subject Cardiotoxin en
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title Isolation of the toxin responsible for gousiekte, a plant-induced cardiomyopathy of ruminants in southern Africa en
dc.type Article en


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