Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)
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Date
Authors
Titoy, G.A.P.
Schwan, Ernst Volker
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Agricultural Research Council, Onderstepoort Veterinary Institute
Abstract
A technique for the cryopreservation of third stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed third-stage larvae in 80% (v/v) ethylene glycol as a cryoprotectant for 30 s at 0°C, prior to transfer into liquid nitrogen. The survival rate, as assessed by motility, was 37,6% after 30 d cryopreservation. A sheep infected percutaneously with 700 live cryopreserved third-stage larvae, harboured 41 adult worms (infectivity rate: 5,8%) when necropsied 78 d later.
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Keywords
Veterinary medicine, Cryopreservation, Sheathed third-stage larvae, Gaigeria pachyscelis, Sandveld hookworm
Sustainable Development Goals
Citation
Titoy, GAP & Schwan, EV 1995, 'Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 1, pp. 63-64.