Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)

dc.contributor.authorTitoy, G.A.P.
dc.contributor.authorSchwan, Ernst Volker
dc.contributor.editorVerwoerd, Daniel Wynand
dc.date.accessioned2013-09-03T10:37:55Z
dc.date.available2013-09-03T10:37:55Z
dc.date.created2013
dc.date.issued1995
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en
dc.description.abstractA technique for the cryopreservation of third stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed third-stage larvae in 80% (v/v) ethylene glycol as a cryoprotectant for 30 s at 0°C, prior to transfer into liquid nitrogen. The survival rate, as assessed by motility, was 37,6% after 30 d cryopreservation. A sheep infected percutaneously with 700 live cryopreserved third-stage larvae, harboured 41 adult worms (infectivity rate: 5,8%) when necropsied 78 d later.en
dc.description.librarianmn2013
dc.identifier.citationTitoy, GAP & Schwan, EV 1995, 'Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 1, pp. 63-64.en
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/22262
dc.language.isoenen
dc.publisherPublished by the Agricultural Research Council, Onderstepoort Veterinary Instituteen
dc.rights© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).en
dc.subjectVeterinary medicineen
dc.subjectCryopreservationen
dc.subjectSheathed third-stage larvaeen
dc.subjectGaigeria pachyscelisen
dc.subjectSandveld hookwormen
dc.subject.lcshVeterinary medicine -- South Africa
dc.titleCryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)en
dc.typeArticleen

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1995 Onderstepoort Journal of Veterinary Research, Volume 62, 1995
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