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Screening of the venoms of two Parabuthus scorpion species on isolated cardiomyocytes

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Date

Authors

Van Aarde, M.N.
Van Rooyen, J.M.
Van Aswegen, G.

Journal Title

Journal ISSN

Volume Title

Publisher

Published by the Agricultural Research Council, Onderstepoort Veterinary Institute

Abstract

Crude, diluted venoms of Parabuthus transvaalicus and P. granulatus were tested on isolated cardiomyocytes. Ventricular myocytes were subjected to hypercontractility tests and whole-cell voltage clamp. Both venoms had a profound influence on the hypercontractility of cardiomyocytes, which was markedly decreased when they were pre-absorbed with commercial antiserum. Whole-cell clamp results showed an increase in the sodium current, and a retardation of the time course of inactivation, implicating the presence of an a toxin in both venoms.

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The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.

Keywords

Veterinary medicine, Cardiomyocytes, Parabuthus granulatus, Parabuthus transvaalicus, Scorpions, Venom

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Citation

Van Aarde, MN, Van Rooyen, JM & Van Aswegen, G 1996, 'Screening of the venoms of two Parabuthus scorpion species on isolated cardiomyocytes’. Onderstepoort Journal of Veterinary Research, vol. 63, no. 2, pp. 127-130.