dc.contributor.author |
Chabalala, Simphiwe
|
|
dc.contributor.author |
Chirwa, Evans M.N.
|
|
dc.date.accessioned |
2013-07-04T14:20:57Z |
|
dc.date.available |
2013-07-04T14:20:57Z |
|
dc.date.issued |
2012 |
|
dc.description.abstract |
Pseudomonas stutzeri, a facultative anaerobe sourced from soil in a uranium mine in Limpopo, South
Africa, reduced uranium-6 (U(VI)) to uranium-4 (U(IV)) in batches under a relatively high initial U(VI)
concentration ranging from 30 to 400 mg/L (pH 5 to 6). U(VI) reduction was rapid during the first 4 to
6 h of incubation followed by slower reduction rates at incubation times longer than 6 hours.
Equilibrium conditions were only obtained after incubation for 24 h. The reduced U(VI) was recovered
in solution as a hydroxide which was determined to consist of U(IV).
A kinetic model based on enzyme kinetics produced the best fit of the optimised model to experimental
data of U(VI) versus time in batch cultures of Pseudomonas stutzeri. The parameters: maximum
specific uranium-6 reduction rate coefficient (ku), half velocity concentration (Ku), and uranium-6
reduction capacity of cells (Tc) were estimated using the data from the 200 mg/L batch. The
parameters obtained in the 200 mg/L batch were then used to simulate the concentration in the other
batches at lower and higher initial U(VI) concentrations. The reduction capacity Tc remained stable but
a loss of accuracy was observed with increasing initial U(VI) concentration.
Numerous batch experiments were conducted to establish kinetic parameters that will be used later for
scale-up purposes. The knowledge gained from such processes will be of practical value in predicting
effluent response to diverse loading conditions. |
en |
dc.description.librarian |
am2013 |
en |
dc.description.librarian |
ai2013 |
en |
dc.description.uri |
http://www.aidic.it/cet |
en |
dc.identifier.citation |
Chabalala S. and Chirwa E., 2012, Modelling U(VI) reduction by Pseudomonas stutzeri, Chemical Engineering Transactions, 27, 265-270 DOI: 10.3303/CET1227045 |
en |
dc.identifier.isbn |
978-88-95608-18-1 |
|
dc.identifier.issn |
1974-9791 |
|
dc.identifier.other |
10.3303/CET1227045 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/21835 |
|
dc.language.iso |
en |
en |
dc.publisher |
AIDIC - The Italian Association of Chemical Engineering |
en |
dc.rights |
© 2012, AIDIC Servizi S.r.l. |
en |
dc.subject |
Pseudomonas stutzeri |
en |
dc.subject |
U(VI) reduction |
en |
dc.subject.lcsh |
Pseudomonas |
en |
dc.subject.lcsh |
Reduction (Chemistry) |
en |
dc.subject.lcsh |
Uranium |
en |
dc.subject.lcsh |
Biochemical engineering |
en |
dc.title |
Modelling U(VI) reduction by Pseudomonas stutzeri |
en |
dc.type |
Article |
en |