The Th1/Th2/Th17 cytokine profile of HIV-infected individuals : a multivariate cytokinomics approach

Show simple item record Williams, Aurelia Alvina Steffens, Francois E. Reinecke, Carolus J. Meyer, Debra 2013-06-04T11:45:18Z 2013-06-04T11:45:18Z 2013-02
dc.description.abstract HIV infection causes the dysregulation of cytokine production. A cytokinomics approach employing cytometric bead array (CBA) technology, flow cytometry and multivariate analysis was applied to the investigation of HIV-induced T helper cell type 1 (Th1), Th2 and Th17 cytokine changes in the serum of treatment naive individuals. Stepwise linear discriminant analysis (LDA) and logistic regression identified interleukin (IL)-6 to be discriminatory for HIV infection with 74.6 and 71.2 % of the cases correctly classified. Analysis of variance (ANOVA) confirmed IL-6 and IL-10 concentrations to be significantly (p= 0.001 and p=0.025) different between the groups. A scatter plot of the log IL-6 and IL-10 concentrations for the groups largely overlapped, with improved differentiation where patients were advancing to the acquired immunodeficiency syndrome (AIDS). IL-17A levels were higher than other cytokines but did not significantly distinguish the groups suggesting that the HIV- and HIV+ individuals had similar immune profiles. This possibility was supported by other clinical indicators. Taken together, the measured cytokines (IL-6, 10 and 17) have potential prognostic value. en_US
dc.description.librarian hb2013 en_US
dc.description.sponsorship This work was supported by the National Research Foundation, Technology Innovation Agency, Medical Research Council and the Faculty of Natural and Agricultural Sciences at the University of Pretoria. en_US
dc.description.uri en_US
dc.identifier.citation Williams, A, Steffens, F, Reinecke, C & Meyer, D 2013, 'The Th1/Th2/Th17 cytokine profile of HIV-infected individuals : a multivariate cytokinomics approach', Cytokine, vol. 61, no. 2, pp. 521-526. en_US
dc.identifier.issn 1043-4666 (print)
dc.identifier.issn 1096-0023 (online)
dc.identifier.other 10.1016/j.cyto.2012.11.006
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Cytokine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cytokine, vol. 61, no. 2, 2013. DOI : 10.1016/j.cyto.2012.11.006 en_US
dc.subject Cytometric bead array en_US
dc.subject Flow cytometry en_US
dc.subject Cytokinomics en_US
dc.subject Interleukin en_US
dc.subject Multivariate en_US
dc.title The Th1/Th2/Th17 cytokine profile of HIV-infected individuals : a multivariate cytokinomics approach en_US
dc.type Postprint Article en_US

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