The Th1/Th2/Th17 cytokine profile of HIV-infected individuals : a multivariate cytokinomics approach

dc.contributor.authorWilliams, Aurelia Alvina
dc.contributor.authorSteffens, Francois E.
dc.contributor.authorReinecke, Carolus J.
dc.contributor.authorMeyer, Debra
dc.contributor.emaildebra.meyer@up.ac.zaen_US
dc.date.accessioned2013-06-04T11:45:18Z
dc.date.available2013-06-04T11:45:18Z
dc.date.issued2013-02
dc.description.abstractHIV infection causes the dysregulation of cytokine production. A cytokinomics approach employing cytometric bead array (CBA) technology, flow cytometry and multivariate analysis was applied to the investigation of HIV-induced T helper cell type 1 (Th1), Th2 and Th17 cytokine changes in the serum of treatment naive individuals. Stepwise linear discriminant analysis (LDA) and logistic regression identified interleukin (IL)-6 to be discriminatory for HIV infection with 74.6 and 71.2 % of the cases correctly classified. Analysis of variance (ANOVA) confirmed IL-6 and IL-10 concentrations to be significantly (p= 0.001 and p=0.025) different between the groups. A scatter plot of the log IL-6 and IL-10 concentrations for the groups largely overlapped, with improved differentiation where patients were advancing to the acquired immunodeficiency syndrome (AIDS). IL-17A levels were higher than other cytokines but did not significantly distinguish the groups suggesting that the HIV- and HIV+ individuals had similar immune profiles. This possibility was supported by other clinical indicators. Taken together, the measured cytokines (IL-6, 10 and 17) have potential prognostic value.en_US
dc.description.librarianhb2013en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation, Technology Innovation Agency, Medical Research Council and the Faculty of Natural and Agricultural Sciences at the University of Pretoria.en_US
dc.description.urihttp://www.journals.elsevier.com/cytokineen_US
dc.identifier.citationWilliams, A, Steffens, F, Reinecke, C & Meyer, D 2013, 'The Th1/Th2/Th17 cytokine profile of HIV-infected individuals : a multivariate cytokinomics approach', Cytokine, vol. 61, no. 2, pp. 521-526.en_US
dc.identifier.issn1043-4666 (print)
dc.identifier.issn1096-0023 (online)
dc.identifier.other10.1016/j.cyto.2012.11.006
dc.identifier.urihttp://hdl.handle.net/2263/21580
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Cytokine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cytokine, vol. 61, no. 2, 2013. DOI : 10.1016/j.cyto.2012.11.006en_US
dc.subjectCytometric bead arrayen_US
dc.subjectFlow cytometryen_US
dc.subjectCytokinomicsen_US
dc.subjectInterleukinen_US
dc.subjectMultivariateen_US
dc.titleThe Th1/Th2/Th17 cytokine profile of HIV-infected individuals : a multivariate cytokinomics approachen_US
dc.typePostprint Articleen_US

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