Determining adrenocortical activity as a measure of stress in African buffalo (Syncerus caffer) based on faecal analysis

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dc.contributor.author Ganswindt, Andre
dc.contributor.author Tordiffe, A.S.W. (Adrian Stephen Wolferstan)
dc.contributor.author Stam, E.
dc.contributor.author Howitt, M.J.
dc.contributor.author Jori, Ferran J.
dc.date.accessioned 2013-03-12T08:29:55Z
dc.date.available 2013-08-22T00:20:04Z
dc.date.issued 2012-08-22
dc.description.abstract Little is known about the levels of stress experienced by African buffalos affected by injury, disease, or socio-ecological and anthropogenic factors. To be able to start filling this gap, we examined the suitability of two 11-oxoaetiocholanolone enzyme-immunoassays (EIAs) detecting 11,17 dioxoandrostanes (11,17-DOA) as well as faecal glucocorticoid metabolites (FGMs) with a 5β-3α-ol-11-one structure (3α,11oxo-CM), respectively, for monitoring stress-related physiological responses in African buffalo. An adrenocorticotrophic hormone (ACTH) challenge in one male and one female housed at Mokopane Biodiversity Conservation Centre, South Africa, showed a threefold increase in circulating cortisol levels in a sample taken 40 min post-injection. Corresponding 11,17-DOA levels increased tenfold (female) and 15-fold (male) above baseline, and 3α,11oxo-CM concentrations increased ninefold (female) and 12-fold (male) above pre-injection levels, indicating that both EIAs are suitable for measuring FGMs in African buffalo.In addition, 11,17-DOA levels monitored during the adaptation process of individual housing revealed an up to 14-fold elevation in FGMs. Storage of faeces at ambient temperature for up to 16 h post-defecation resulted in an significant increase in 11,17-DOA levels 2 h after defecation. Finally, higher individual baseline 11,17-DOA concentrations were found in samples defecated overnight, indicating a possible diurnal effect in excretion of FGMs in African buffalo. en
dc.description.librarian am2013 en
dc.description.librarian ab2013
dc.description.uri http://africanzoology.journals.ac.za/ en
dc.identifier.citation Ganswindt, A, Tordiffe, ASW, Stam, E, Howitt, MJ & Jori, F 2012, 'Determining adrenocortical activity as a measure of stress in African buffalo (Syncerus caffer) based on faecal analysis', African Zoology, vol. 47, no. 2, pp. 261-269. en
dc.identifier.issn 1562-7020 (print)
dc.identifier.issn 2224-073X (online)
dc.identifier.other 6507085906
dc.identifier.other G-9856-2014
dc.identifier.uri http://hdl.handle.net/2263/21168
dc.language.iso en en
dc.publisher Zoological Society of Southern Africa en
dc.rights SU LIS. en
dc.subject Faecal glucocorticoid metabolite (fGCM) en
dc.subject ACTH challenge test en
dc.subject Animal separation en
dc.subject Hormone degradation rate en
dc.subject Circadian variation en
dc.subject.lcsh African buffalo -- Effect of stress on en
dc.subject.lcsh Feces en
dc.subject.lcsh Adrenocortical hormones en
dc.title Determining adrenocortical activity as a measure of stress in African buffalo (Syncerus caffer) based on faecal analysis en
dc.type Article en


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