Determining adrenocortical activity as a measure of stress in African buffalo (Syncerus caffer) based on faecal analysis

dc.contributor.authorGanswindt, Andre
dc.contributor.authorTordiffe, Adrian Stephen Wolferstan
dc.contributor.authorStam, E.
dc.contributor.authorHowitt, M.J.
dc.contributor.authorJori, Ferran J.
dc.contributor.emailaganswindt@zoology.up.ac.zaen
dc.date.accessioned2013-03-12T08:29:55Z
dc.date.available2013-08-22T00:20:04Z
dc.date.issued2012-08-22
dc.description.abstractLittle is known about the levels of stress experienced by African buffalos affected by injury, disease, or socio-ecological and anthropogenic factors. To be able to start filling this gap, we examined the suitability of two 11-oxoaetiocholanolone enzyme-immunoassays (EIAs) detecting 11,17 dioxoandrostanes (11,17-DOA) as well as faecal glucocorticoid metabolites (FGMs) with a 5β-3α-ol-11-one structure (3α,11oxo-CM), respectively, for monitoring stress-related physiological responses in African buffalo. An adrenocorticotrophic hormone (ACTH) challenge in one male and one female housed at Mokopane Biodiversity Conservation Centre, South Africa, showed a threefold increase in circulating cortisol levels in a sample taken 40 min post-injection. Corresponding 11,17-DOA levels increased tenfold (female) and 15-fold (male) above baseline, and 3α,11oxo-CM concentrations increased ninefold (female) and 12-fold (male) above pre-injection levels, indicating that both EIAs are suitable for measuring FGMs in African buffalo.In addition, 11,17-DOA levels monitored during the adaptation process of individual housing revealed an up to 14-fold elevation in FGMs. Storage of faeces at ambient temperature for up to 16 h post-defecation resulted in an significant increase in 11,17-DOA levels 2 h after defecation. Finally, higher individual baseline 11,17-DOA concentrations were found in samples defecated overnight, indicating a possible diurnal effect in excretion of FGMs in African buffalo.en
dc.description.librarianam2013en
dc.description.librarianab2013
dc.description.urihttp://africanzoology.journals.ac.za/en
dc.identifier.citationGanswindt, A, Tordiffe, ASW, Stam, E, Howitt, MJ & Jori, F 2012, 'Determining adrenocortical activity as a measure of stress in African buffalo (Syncerus caffer) based on faecal analysis', African Zoology, vol. 47, no. 2, pp. 261-269.en
dc.identifier.issn1562-7020 (print)
dc.identifier.issn2224-073X (online)
dc.identifier.other6507085906
dc.identifier.otherG-9856-2014
dc.identifier.urihttp://hdl.handle.net/2263/21168
dc.language.isoenen
dc.publisherZoological Society of Southern Africaen
dc.rightsSU LIS.en
dc.subjectFaecal glucocorticoid metabolite (fGCM)en
dc.subjectACTH challenge testen
dc.subjectAnimal separationen
dc.subjectHormone degradation rateen
dc.subjectCircadian variationen
dc.subject.lcshAfrican buffalo -- Effect of stress onen
dc.subject.lcshFecesen
dc.subject.lcshAdrenocortical hormonesen
dc.titleDetermining adrenocortical activity as a measure of stress in African buffalo (Syncerus caffer) based on faecal analysisen
dc.typeArticleen

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