dc.contributor.author |
Wang, Zheng-Wei
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|
dc.contributor.author |
Vreede, F.T.
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|
dc.contributor.author |
Mitchell, J.O.
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|
dc.contributor.author |
Viljoen, G.J.
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|
dc.contributor.editor |
Boomker, Jacob Diederik Frederik |
|
dc.date.accessioned |
2012-12-12T06:35:45Z |
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dc.date.available |
2012-12-12T06:35:45Z |
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dc.date.created |
2012 |
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dc.date.issued |
2001 |
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dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en_US |
dc.description.abstract |
A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein gene-specific RNA from virulent, avirulent or all isolates respectively. The sensitivity of one-step RT-PCR was determined using viral RNA extracted from serially diluted NDV-infected allantoic fluid and found to be 10ˉ⁵ HA units. Application of one-step RT-PCR to various NDV samples, including wild-type virulent isolates and avirulent vaccine strains, demonstrated the potential for rapid identification (3-4 h) of NDV isolates as well as the differentiation of virulent from avirulent strains. |
en_US |
dc.identifier.citation |
Wang, Z, Vreede, FT, Mitchell, JO & Viljoen, GJ 2001, 'Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 2, pp. 131-134. |
en_US |
dc.identifier.issn |
0030-2465 |
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dc.identifier.uri |
http://hdl.handle.net/2263/20686 |
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dc.language |
en |
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dc.publisher |
Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria. |
en_US |
dc.rights |
© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original).
© University of Pretoria. Dept of Library Services (digital). |
en_US |
dc.subject |
Veterinary medicine |
en_US |
dc.subject |
Detection |
en_US |
dc.subject |
Identification |
en_US |
dc.subject |
Newcastle disease virus (NDV) |
en_US |
dc.subject |
RT-PCR |
en_US |
dc.subject |
Triton X-100 differentiation of NDV isolates |
en_US |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.title |
Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR |
en_US |
dc.type |
Article |
en_US |