Selection of an scFv phage antibody that recognizes bluetongue virus from a large synthetic library and its use in ELISAs to detect viral antigen and antibodies

Van Wyngaardt, Wouter; Du Plessis, D.H.

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1998 Onderstepoort Journal of Veterinary Research, Volume 65, 1998
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dc.contributor.author	Van Wyngaardt, Wouter
dc.contributor.author	Du Plessis, D.H.
dc.contributor.editor	Verwoerd, Daniel Wynand
dc.date.accessioned	2012-10-17T09:30:39Z
dc.date.available	2012-10-17T09:30:39Z
dc.date.created	2012
dc.date.issued	1998
dc.description	The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en
dc.description.abstract	A filamentous phage library displaying a vast repertoire of synthetic single chain fragment variable (scFv) antibody fragments was subjected to affinity selection on purified bluetongue virus (BTV) particles. After four rounds of selection and amplification, 73 out of a total of 90 fusion phage clones tested were found to bind to purified BTV in ELISA. One of these, the clone producing the highest ELISA signal, was selected for an investigation of its potential as an immunodiagnostic reagent. The binding of this phage antibody (designated A12) could be inhibited by free virus and by antibodies in immune serum. Inhibition with antibodies in guinea-pig sera suggested that it recognized an antigenic region on BTV that was similar on at least 10 different BTV serotypes. A sandwich ELISA utilizing antibody A12 was capable of detecting approximately 60 ng of purified BTV.	en
dc.description.librarian	mn2012	en
dc.identifier.citation	Van Wyngaardt, W & Du Plessis, DH 1998, 'Selection of an scFv phage antibody that recognizes bluetongue virus from a large synthetic library and its use in ELISAs to detect viral antigen and antibodies’. Onderstepoort Journal of Veterinary Research, vol. 65, no. 2, pp. 125-131.	en
dc.identifier.issn	0330-2465
dc.identifier.uri	http://hdl.handle.net/2263/20199
dc.language.iso	en	en
dc.publisher	Published by the Agricultural Research Council, Onderstepoort Veterinary Institute	en
dc.rights	© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).	en
dc.subject	Veterinary medicine	en
dc.subject	Bluetongue virus	en
dc.subject	ELISA	en
dc.subject	Phage antibodies	en
dc.subject	Synthetic library	en
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.subject.lcsh	Veterinary virology	en
dc.subject.lcsh	Bluetongue -- South Africa	en
dc.title	Selection of an scFv phage antibody that recognizes bluetongue virus from a large synthetic library and its use in ELISAs to detect viral antigen and antibodies	en
dc.type	Article	en