Selection of an scFv phage antibody that recognizes bluetongue virus from a large synthetic library and its use in ELISAs to detect viral antigen and antibodies
| dc.contributor.author | Van Wyngaardt, Wouter | |
| dc.contributor.author | Du Plessis, D.H. | |
| dc.contributor.editor | Verwoerd, Daniel Wynand | |
| dc.date.accessioned | 2012-10-17T09:30:39Z | |
| dc.date.available | 2012-10-17T09:30:39Z | |
| dc.date.created | 2012 | |
| dc.date.issued | 1998 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
| dc.description.abstract | A filamentous phage library displaying a vast repertoire of synthetic single chain fragment variable (scFv) antibody fragments was subjected to affinity selection on purified bluetongue virus (BTV) particles. After four rounds of selection and amplification, 73 out of a total of 90 fusion phage clones tested were found to bind to purified BTV in ELISA. One of these, the clone producing the highest ELISA signal, was selected for an investigation of its potential as an immunodiagnostic reagent. The binding of this phage antibody (designated A12) could be inhibited by free virus and by antibodies in immune serum. Inhibition with antibodies in guinea-pig sera suggested that it recognized an antigenic region on BTV that was similar on at least 10 different BTV serotypes. A sandwich ELISA utilizing antibody A12 was capable of detecting approximately 60 ng of purified BTV. | en |
| dc.description.librarian | mn2012 | en |
| dc.identifier.citation | Van Wyngaardt, W & Du Plessis, DH 1998, 'Selection of an scFv phage antibody that recognizes bluetongue virus from a large synthetic library and its use in ELISAs to detect viral antigen and antibodies’. Onderstepoort Journal of Veterinary Research, vol. 65, no. 2, pp. 125-131. | en |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/20199 | |
| dc.language.iso | en | en |
| dc.publisher | Published by the Agricultural Research Council, Onderstepoort Veterinary Institute | en |
| dc.rights | © ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital). | en |
| dc.subject | Veterinary medicine | en |
| dc.subject | Bluetongue virus | en |
| dc.subject | ELISA | en |
| dc.subject | Phage antibodies | en |
| dc.subject | Synthetic library | en |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.subject.lcsh | Veterinary virology | en |
| dc.subject.lcsh | Bluetongue -- South Africa | en |
| dc.title | Selection of an scFv phage antibody that recognizes bluetongue virus from a large synthetic library and its use in ELISAs to detect viral antigen and antibodies | en |
| dc.type | Article | en |