dc.contributor.author |
Du Plessis, D.H.
|
|
dc.contributor.author |
Van Wyngaardt, Wouter
|
|
dc.contributor.author |
Romito, M.
|
|
dc.contributor.author |
Du Plessis, M.
|
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dc.contributor.editor |
Verwoerd, Daniel Wynand |
|
dc.contributor.upauthor |
Maree, Sarita
|
|
dc.date.accessioned |
2012-10-01T11:48:59Z |
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dc.date.available |
2012-10-01T11:48:59Z |
|
dc.date.created |
2012 |
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dc.date.issued |
1999 |
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dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en |
dc.description.abstract |
An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV)
was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody
specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk
derived chicken lgY directed against AHSV (serotype 3) was used as the secondary antibody. Since
lgY and mouse lgG do not cross-react serologically, the secondary antibody was not labelled, but
was instead detected with enzyme-coupled sheep antibodies directed against avian immunoglobulins.
The assay recognized all nine AHSV serotypes, but not the Cascara isolate of equine encephalosis
virus, a related orbivirus that also infects horses. In addition to being able to detect and quantify whole
AHSV, the ELISA could show the presence of VP7 produced by recombinant baculoviruses. |
en |
dc.description.librarian |
mn2012 |
en |
dc.identifier.citation |
Du Plessis, DH, Van Wyngaardt, W, Romito, M, Du Plessis, M & Maree, S 1999, 'The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus’. Onderstepoort Journal of Veterinary Research, vol. 66, no. 1, pp. 25-28. |
en |
dc.identifier.issn |
0330-2465 |
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dc.identifier.uri |
http://hdl.handle.net/2263/19926 |
|
dc.language.iso |
en |
en |
dc.publisher |
Pretoria : Agricultural Research Council, Onderstepoort Veterinary Institute |
en |
dc.rights |
©ARC-Onderstepoort (original). ©University of Pretoria. Dept of Library Services (digital). |
en |
dc.subject |
African horse sickness |
en |
dc.subject |
Antibody sandwich |
en |
dc.subject |
Chickens |
en |
dc.subject |
Egg-yolk |
en |
dc.subject |
Orbiviruses |
en |
dc.subject |
AHS |
|
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.subject.lcsh |
African horse sickness -- South Africa |
en |
dc.subject.lcsh |
Veterinary virology |
en |
dc.subject.lcsh |
Horses -- Diseases |
en_ZA |
dc.title |
The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus |
en |
dc.type |
Article |
en |