Synergistic antimycobacterial actions of knowltonia vesicatoria (L.f) sims

Show simple item record Labuschagne, Antoinette Hussein, Ahmed A. Rodriguez, Benjamin Lall, Namrita 2012-07-04T08:50:27Z 2012-07-04T08:50:27Z 2012
dc.description This paper is part of the thesis submitted by the first author to the Department of Plant Science of University of Pretoria. en_US
dc.description.abstract Euclea natalensis A.DC., Knowltonia vesicatoria (L.f) Sims, and Pelargonium sidoides DC. are South African plants traditionally used to treat tuberculosis. Extracts from these plants were used in combination with isoniazid (INH) to investigate the possibility of synergy with respect to antimycobacterial activity. The ethanol extract of K. vesicatoria was subjected to fractionation to identify the active compounds. The activity of the Knowltonia extract remained superior to the fractions with a minimum inhibitory concentration (MIC) of 625.0 μg/mL against Mycobacterium smegmatis and an MIC of 50.00 μg/mL against M. tuberculosis. The K. vesicatoria extract was tested against two different drug-resistant strains of M. tuberculosis, which resulted in an MIC of 50.00 μg/mL on both strains. The combination of K. vesicatoria with INH exhibited the best synergistic antimycobacterial activity with a fractional inhibitory concentration index of 0.25 (a combined concentration of 6.28 μg/mL). A fifty percent inhibitory concentration of this combination against U937 cells was 121.0 μg/mL. Two compounds, stigmasta-5,23-dien-3-ol (1) and 5- (hydroxymethyl)furan-2(5H)-one (2), were isolated from K. vesicatoria as the first report of isolation for both compounds from this plant and the first report of antimycobacterial activity. Compound (1) was active against drug-sensitive M. tuberculosis with an MIC of 50.00 μg/mL. en
dc.description.librarian nf2012 en
dc.description.sponsorship The National Research Foundation en_US
dc.description.uri en_US
dc.identifier.citation Labuschagne, A, Hussein, AA, Rodrıguez, B & Lall, N 2012, 'Synergistic antimycobacterial actions of knowltonia vesicatoria (L.f) sims', Evidence-Based Complementary and Alternative Medicine, vol. 2012, no. ID 808979, pp. 1-9. en
dc.identifier.issn 1741-4288 (online)
dc.identifier.issn 1741-427X (print)
dc.identifier.other 10.1155/2012/808979
dc.language.iso en en_US
dc.publisher Hindawi Publishing Corporation en_US
dc.rights Copyright © 2012 Antoinette Labuschagn´e et al. This is an open access article distributed under the Creative Commons Attribution License. en_US
dc.subject Antimycobacterial activity en
dc.subject.lcsh Antibacterial agents en
dc.subject.lcsh Tuberculosis -- Alternative treatment -- South Africa en
dc.subject.lcsh Plant extracts -- Therapeutic use -- South Africa en
dc.title Synergistic antimycobacterial actions of knowltonia vesicatoria (L.f) sims en
dc.type Article en

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