Synergistic antimycobacterial actions of knowltonia vesicatoria (L.f) sims

dc.contributor.authorLabuschagne, Antoinette
dc.contributor.authorHussein, Ahmed A.
dc.contributor.authorRodriguez, Benjamin
dc.contributor.authorLall, Namrita
dc.contributor.emailnamrita.lall@up.ac.zaen_US
dc.date.accessioned2012-07-04T08:50:27Z
dc.date.available2012-07-04T08:50:27Z
dc.date.issued2012
dc.descriptionThis paper is part of the thesis submitted by the first author to the Department of Plant Science of University of Pretoria.en_US
dc.description.abstractEuclea natalensis A.DC., Knowltonia vesicatoria (L.f) Sims, and Pelargonium sidoides DC. are South African plants traditionally used to treat tuberculosis. Extracts from these plants were used in combination with isoniazid (INH) to investigate the possibility of synergy with respect to antimycobacterial activity. The ethanol extract of K. vesicatoria was subjected to fractionation to identify the active compounds. The activity of the Knowltonia extract remained superior to the fractions with a minimum inhibitory concentration (MIC) of 625.0 μg/mL against Mycobacterium smegmatis and an MIC of 50.00 μg/mL against M. tuberculosis. The K. vesicatoria extract was tested against two different drug-resistant strains of M. tuberculosis, which resulted in an MIC of 50.00 μg/mL on both strains. The combination of K. vesicatoria with INH exhibited the best synergistic antimycobacterial activity with a fractional inhibitory concentration index of 0.25 (a combined concentration of 6.28 μg/mL). A fifty percent inhibitory concentration of this combination against U937 cells was 121.0 μg/mL. Two compounds, stigmasta-5,23-dien-3-ol (1) and 5- (hydroxymethyl)furan-2(5H)-one (2), were isolated from K. vesicatoria as the first report of isolation for both compounds from this plant and the first report of antimycobacterial activity. Compound (1) was active against drug-sensitive M. tuberculosis with an MIC of 50.00 μg/mL.en
dc.description.librariannf2012en
dc.description.sponsorshipThe National Research Foundationen_US
dc.description.urihttp://www.hindawi.com/journals/ecam/en_US
dc.identifier.citationLabuschagne, A, Hussein, AA, Rodrıguez, B & Lall, N 2012, 'Synergistic antimycobacterial actions of knowltonia vesicatoria (L.f) sims', Evidence-Based Complementary and Alternative Medicine, vol. 2012, no. ID 808979, pp. 1-9.en
dc.identifier.issn1741-4288 (online)
dc.identifier.issn1741-427X (print)
dc.identifier.other10.1155/2012/808979
dc.identifier.urihttp://hdl.handle.net/2263/19322
dc.language.isoenen_US
dc.publisherHindawi Publishing Corporationen_US
dc.rightsCopyright © 2012 Antoinette Labuschagn´e et al. This is an open access article distributed under the Creative Commons Attribution License.en_US
dc.subjectAntimycobacterial activityen
dc.subject.lcshAntibacterial agentsen
dc.subject.lcshTuberculosis -- Alternative treatment -- South Africaen
dc.subject.lcshPlant extracts -- Therapeutic use -- South Africaen
dc.titleSynergistic antimycobacterial actions of knowltonia vesicatoria (L.f) simsen
dc.typeArticleen

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