Expression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl millet

Show simple item record O'Kennedy, M.M. Crampton, Bridget Genevieve Lorito, M. Chakauya, E. Breese, W.A. Burger, J.T. Botha, F.C. 2012-05-02T08:54:35Z 2012-05-02T08:54:35Z 2011-04
dc.description.abstract Sclerospora graminicola is an oomycete (heterotrophic Stramenopiles), fungal-like obligate phytopathogen, the causal agent of downy mildew in pearl millet (Pennisetum glaucum [L.] R. Br.), and a major constraint in the production of this cereal crop. In this study a hydrolytic enzyme, β-1,3-glucanase (gluc78), from the biocontrol fungus Trichoderma atroviride, was introduced into the genome of a pearl millet breeding line, 842B, by particle bombardment. Constructs were prepared containing the gluc78 gene, encoding the 78 kDa β-1,3-glucanase protein, downstream of either the constitutive ubiquitin promoter or the wound inducible potato proteinase inhibitor IIK gene promoter (pin2). The positive selectable marker gene, manA, encoding mannose-6-phosphate isomerase (phosphomannose isomerase) under the control of the ubiquitin promoter, was used for co-transformation. Transgenic plants were obtained harbouring the manA selectable marker gene and the antifungal gene gluc78 downstream of either the ubiquitin or pin2 promoter. Full constructs or minimal transgene expression cassettes containing the genes of interest were successfully introduced into the genome of pearl millet. Progeny of stably transformed plants, harbouring the gluc78 transgene which is driven by the pin2 promoter and followed by the rice Act1 intron sequences, was subjected to pathogenicity trials. One transgenic event exhibited a reduction of 58% in the incidence of S. graminicola infection, however other transgenic pearl millet events showed no resistance to this phytopathogen. The event conferring decreased susceptibility to S. graminicola had high levels of the glucanase transcript especially in transgenic plants showing higher levels of downy mildew infection. en
dc.description.librarian nf2012 en
dc.description.sponsorship The European Commission contract number: ERBIC18 CT98 0316. Some results presented in this document are outputs from several research projects of the Plant Sciences Research Programme funded by the UK Department for International Development (DFID) and administered by the Centre for Arid Zone Studies (CAZS) for the benefit of developing countries. en_US
dc.description.uri en_US
dc.identifier.citation MM O'Kennedy, BG Crampton, M Lorito, E Chakauya, WA Breese, JT Burger & FC Botha, Expression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl millet, South African Journal of Botany, vol. 77, no. 2, pp. 335-345 (2011), doi: 10.1016/j.sajb.2010.09.016. en
dc.identifier.issn 0254-6299 (print)
dc.identifier.issn 1727-9321 (online)
dc.identifier.other 10.1016/j.sajb.2010.09.016
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2010 SAAB. Published by Elsevier B.V. All rights reserved. en_US
dc.subject Fungal resistance en
dc.subject Sclerospora graminicola en
dc.subject Transgenic pearl millet en
dc.subject β-1,3-glucanase en
dc.subject Trichoderma atroviride en
dc.subject.lcsh Transgenic plants en
dc.subject.lcsh Pearl millet -- Diseases and pests -- Biological control en
dc.subject.lcsh Downy mildew diseases en
dc.subject.lcsh Fungi as biological pest control agents en
dc.title Expression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl millet en
dc.type Postprint Article en

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