Expression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl millet

dc.contributor.authorO'Kennedy, M.M.
dc.contributor.authorCrampton, Bridget Genevieve
dc.contributor.authorLorito, M.
dc.contributor.authorChakauya, E.
dc.contributor.authorBreese, W.A.
dc.contributor.authorBurger, J.T.
dc.contributor.authorBotha, F.C.
dc.date.accessioned2012-05-02T08:54:35Z
dc.date.available2012-05-02T08:54:35Z
dc.date.issued2011-04
dc.description.abstractSclerospora graminicola is an oomycete (heterotrophic Stramenopiles), fungal-like obligate phytopathogen, the causal agent of downy mildew in pearl millet (Pennisetum glaucum [L.] R. Br.), and a major constraint in the production of this cereal crop. In this study a hydrolytic enzyme, β-1,3-glucanase (gluc78), from the biocontrol fungus Trichoderma atroviride, was introduced into the genome of a pearl millet breeding line, 842B, by particle bombardment. Constructs were prepared containing the gluc78 gene, encoding the 78 kDa β-1,3-glucanase protein, downstream of either the constitutive ubiquitin promoter or the wound inducible potato proteinase inhibitor IIK gene promoter (pin2). The positive selectable marker gene, manA, encoding mannose-6-phosphate isomerase (phosphomannose isomerase) under the control of the ubiquitin promoter, was used for co-transformation. Transgenic plants were obtained harbouring the manA selectable marker gene and the antifungal gene gluc78 downstream of either the ubiquitin or pin2 promoter. Full constructs or minimal transgene expression cassettes containing the genes of interest were successfully introduced into the genome of pearl millet. Progeny of stably transformed plants, harbouring the gluc78 transgene which is driven by the pin2 promoter and followed by the rice Act1 intron sequences, was subjected to pathogenicity trials. One transgenic event exhibited a reduction of 58% in the incidence of S. graminicola infection, however other transgenic pearl millet events showed no resistance to this phytopathogen. The event conferring decreased susceptibility to S. graminicola had high levels of the glucanase transcript especially in transgenic plants showing higher levels of downy mildew infection.en
dc.description.librariannf2012en
dc.description.sponsorshipThe European Commission contract number: ERBIC18 CT98 0316. Some results presented in this document are outputs from several research projects of the Plant Sciences Research Programme funded by the UK Department for International Development (DFID) and administered by the Centre for Arid Zone Studies (CAZS) for the benefit of developing countries.en_US
dc.description.urihttp://www.elsevier.com/locate/sajben_US
dc.identifier.citationMM O'Kennedy, BG Crampton, M Lorito, E Chakauya, WA Breese, JT Burger & FC Botha, Expression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl millet, South African Journal of Botany, vol. 77, no. 2, pp. 335-345 (2011), doi: 10.1016/j.sajb.2010.09.016.en
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2010.09.016
dc.identifier.urihttp://hdl.handle.net/2263/18657
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2010 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.subjectFungal resistanceen
dc.subjectSclerospora graminicolaen
dc.subjectTransgenic pearl milleten
dc.subjectβ-1,3-glucanaseen
dc.subjectTrichoderma atrovirideen
dc.subject.lcshTransgenic plantsen
dc.subject.lcshPearl millet -- Diseases and pests -- Biological controlen
dc.subject.lcshDowny mildew diseasesen
dc.subject.lcshFungi as biological pest control agentsen
dc.titleExpression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl milleten
dc.typePostprint Articleen

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