The Hybrid II assay : a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle
Loading...
Date
Authors
Pienaar, Ronel
Potgieter, Fred T.
Latif, Abdalla A.
Thekisoe, Oriel M.M.
Mans, Ben J. (Barend Johannes)
Journal Title
Journal ISSN
Volume Title
Publisher
Cambridge University Press
Abstract
Corridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria parva. This is a nationally controlled disease in South Africa and strict control measures apply for the movement of buffalo, which includes mandatory testing for the presence of T. parva and other controlled diseases. Accurate diagnosis of the T. parva carrier state in buffalo using the official real-time hybridization PCR assay (Sibeko et al. 2008), has been shown to be affected by concurrent infection with T. sp. (buffalo)-like parasites.We describe the Hybrid II assay, a real-time hybridization PCR method, which compareswell with the official hybridization assay in terms of specificity and sensitivity. It is, however, not influenced by mixed infections of T. sp. (buffalo)-like parasites and is as such a significant improvement on the current hybridization assay.
Description
Keywords
Corridor disease, Diagnostic assay, Real-time hybridization, Syncerus caffer
Sustainable Development Goals
Citation
Pienaar, R, Potgieter, FT, Latif, AA, Thekisoe, OMM & Mans, BJ 2011, 'The Hybrid II assay : a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle', Parasitology, vol. 138, no. 14, pp. 1935-1944.