The Hybrid II assay : a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle

dc.contributor.authorPienaar, Ronel
dc.contributor.authorPotgieter, Fred T.
dc.contributor.authorLatif, Abdalla A.
dc.contributor.authorThekisoe, Oriel M.M.
dc.contributor.authorMans, Ben J. (Barend Johannes)
dc.date.accessioned2012-03-05T13:09:43Z
dc.date.available2012-09-09T00:20:03Z
dc.date.issued2011-09-09
dc.description.abstractCorridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria parva. This is a nationally controlled disease in South Africa and strict control measures apply for the movement of buffalo, which includes mandatory testing for the presence of T. parva and other controlled diseases. Accurate diagnosis of the T. parva carrier state in buffalo using the official real-time hybridization PCR assay (Sibeko et al. 2008), has been shown to be affected by concurrent infection with T. sp. (buffalo)-like parasites.We describe the Hybrid II assay, a real-time hybridization PCR method, which compareswell with the official hybridization assay in terms of specificity and sensitivity. It is, however, not influenced by mixed infections of T. sp. (buffalo)-like parasites and is as such a significant improvement on the current hybridization assay.en
dc.description.librarianab2012en
dc.description.sponsorshipThis project was funded by OVI project numbers 15/08/ 1P01 and OV21/03/C148 (Department of Agriculture, Forestry and Fisheries contract).en
dc.description.urihttp://journals.cambridge.org/action/displayJournal?jid=paren
dc.identifier.citationPienaar, R, Potgieter, FT, Latif, AA, Thekisoe, OMM & Mans, BJ 2011, 'The Hybrid II assay : a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle', Parasitology, vol. 138, no. 14, pp. 1935-1944.en
dc.identifier.issn0031-1820
dc.identifier.otherDOI:10.1017/S0031182011001454
dc.identifier.urihttp://hdl.handle.net/2263/18366
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.relation.requiresAdobe Acrobat Readeren
dc.rights© Cambridge University Press 2011. This article is embargoed by the piblisher until 09 September 2012.en
dc.subjectCorridor diseaseen
dc.subjectDiagnostic assayen
dc.subjectReal-time hybridizationen
dc.subjectSyncerus cafferen
dc.subject.lcshTheileria parvaen
dc.subject.lcshAfrican buffaloen
dc.subject.lcshCattle -- Diseasesen
dc.subject.lcshHybridizationen
dc.titleThe Hybrid II assay : a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattleen
dc.typeArticleen

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