Abstract:
The present study was undertaken to explore the possible biochemical activities of Hyaenanche globosa Lamb. and its
compounds. Two different extracts (ethanol and dichloromethane) of four different parts (leaves, root, stem, and fruits)
of H. globosa were evaluated for their possible antibacterial, antityrosinase, and anticancer (cytotoxicity) properties. Two
pure compounds were isolated using column chromatographic techniques. Active extracts and pure compounds were
investigated for their antioxidant effect on cultured ‘Hela cells’. Antioxidant/oxidative properties of the ethanolic extract
of the fruits of H. globosa and purifi ed compounds were investigated using reactive oxygen species (ROS), ferric-reducing
antioxidant power (FRAP), and lipid peroxidation thiobarbituric acid reactive substance (TBARS) assays. The ethanolic
extract of the leaves and fruits of H. globosa showed the best activity, exhibiting a minimum inhibitory concentration (MIC)
of 3.1 mg/ ml and a minimum bactericidal concentration (MBC) of 1.56 and 6.2 mg/ml, respectively, against M. smegmatis.
The ethanolic extract of the fruits of H. globosa (F.E) showed the highest percentage of inhibitory activity of monophenolase
(90.4% at 200 g/ml). In addition, F.E exhibited 50% inhibitory concentration (IC50) of 37.7 g/ml on the viability of ‘HeLa
cells’ using cytotoxicity MTT assay. Subsequently, F.E was fractionated using phase-partitioning with n-hexane, ethyl
acetate, and n-butanol. The cytotoxicity of these fractions were determined in vitro using different cancer cell lines. The
n-hexane fraction exhibited the highest activity of toxicity. Therefore, this fraction was subjected to further separation by
chromatographic methods. Two pure compounds known as: ‘Tutin’ and ‘hyenanchin’ were isolated and their structures
were determined by NMR spectroscopic methods. Unpredictably, none of them showed signifi cant (P 0.01) inhibition on
cell viability/proliferation at the concentrations that were used. F.E showed significant anti-tyrosinase, antibacterial, and
cytotoxicity effects, therefore it can be considered as an effective inhibitor alone or in combination with other plant extracts.
