The seven species and one subspecies, at present attributed to Spilophorus in the Afrotropical region, are revised. One new species, Spilophorus pringlei sp. n., and one new subspecies, S. plagosus vrystaati subsp. n., are described. A key and illustrations to the species and subspecies are provided. The male of S. grandis Schein, 1949 is described for the first time. A new subgenus, Spilophorus (Prospilophorus) subgen. n., is established.