BACKGROUND: The cattle tick, Rhipicephalus (Boophilus) microplus, economically impact cattle industry in tropical
and subtropical regions of the world. The morphological and genetic differences among R. microplus strains have
been documented in the literature, suggesting that biogeographical and ecological separation may have resulted
in boophilid ticks from America/Africa and those from Australia being different species. To test the hypothesis of
the presence of different boophilid species, herein we performed a series of experiments to characterize the
reproductive performance of crosses between R. microplus from Australia, Africa and America and the genetic
diversity of strains from Australia, Asia, Africa and America.
RESULTS: The results showed that the crosses between Australian and Argentinean or Mozambican strains of
boophilid ticks are infertile while crosses between Argentinean and Mozambican strains are fertile. These results
showed that tick strains from Africa (Mozambique) and America (Argentina) are the same species, while ticks
from Australia may actually represent a separate species. The genetic analysis of mitochondrial 12S and 16S rDNA
and microsatellite loci were not conclusive when taken separately, but provided evidence that Australian tick
strains were genetically different from Asian, African and American strains.
CONCLUSION: The results reported herein support the hypothesis that at least two different species share the
name R. microplus. These species could be redefined as R. microplus (Canestrini, 1887) (for American and African
strains) and probably the old R. australis Fuller, 1899 (for Australian strains), which needs to be redescribed.
However, experiments with a larger number of tick strains from different geographic locations are needed to
corroborate these results.