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Browsing by UP Author "Huismans, H. (Henk), 1942-"
Now showing items 1-20 of 27
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Stassen, Liesel; Huismans, H. (Henk), 1942-; Theron, Jacques (Elsevier, 2012-01)Infection of mammalian cell cultures with African horse sickness virus (AHSV) is known to result in dramatic cytopathic effects (CPE), but no CPE is observed in infected insect cell cultures despite productive virus ...
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Huismans, H. (Henk), 1942-; Weiss, K.E. (Published by the Government Printer, Pretoria, 1969)Bluetongue virus was found to induce interferon in mouse embryo (ME) cells and in mice. Different strains of bluetongue virus differed in their ability to induce interferon. Interferon production in ME cells commences after ...
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Venter, Eduard; Van der Merwe, Christiaan F.; Buys, A.V. (Antonia Vergina); Huismans, H. (Henk), 1942-; Van Staden, Vida (Society for General Microbiology, 2014-03)African horse sickness virus (AHSV) is an arbovirus capable of successfully replicating in both its mammalian host and insect vector. Where mammalian cells show a severe cytopathic effect (CPE) following AHSV infection, ...
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Huismans, H. (Henk), 1942-; Bremer, C.W.; Steyn, P.J.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Published by The Government Printer, Pretoria, 1981)No major differences in size were observed when both the double-stranded RNA and the polypeptides of the Australian bluetongue virus (BTV) isolate CSIRO 19 (BTV-20) were compared with those of other BTV serotypes such as ...
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Venter, Estelle Hildegard; Huismans, H. (Henk), 1942-; Van Dijk, A.A.; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1995)The serogroup specificity of the bluetongue virus (BTV) NS1 and VP3 gene probes was confirmed by means of northern blot hybridization. Under high-stringency conditions both probes hybridized to 22 BTV serotypes (18 South ...
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Wall, Gayle V.; Rutkowska, Daria Anna; Mizrachi, Eshchar; Huismans, H. (Henk), 1942-; Van Staden, Vida (Cambridge University Press, 2017-02)The bulk of African horse sickness virus (AHSV) major core protein VP7 self-assembles into flat, hexagonal crystalline particles in a process appearing unrelated to viral replication. Why this unique characteristic of AHSV ...
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Huismans, H. (Henk), 1942-; Van Dijk, A.A.; Steyn, P.J.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Jordaan, E.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Published by The Government Printer, Pretoria, 1982)Virions of bluetongue virus (BTV), epizootic haemorrhagic disease virus (EHDV) and African horsesickness virus (AHSV) can be converted to core particles by treatment with chymotrypsin and magnesium. The conversion is ...
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Pretorius, Jakobus Maree; Huismans, H. (Henk), 1942-; Theron, Jacques (Elsevier, 2015-12)Bluetongue virus (BTV), the type species of the genus Orbivirus within the family Reoviridae, has a genome consisting of 10 linear double-stranded RNA genome segments. Current reverse genetics approaches for engineering ...
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Conradie, Andele M.; Stassen, Liesel; Huismans, H. (Henk), 1942-; Potgieter, Christiaan A.; Theron, Jacques (Elsevier, 2016-12)In an effort to simplify and expand the utility of African horse sickness virus (AHSV) reverse genetics, different plasmid-based reverse genetics systems were developed. Plasmids containing cDNAs corresponding to each of ...
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Bekker, Shani; Huismans, H. (Henk), 1942-; Van Staden, Vida (Elsevier, 2014-05)African horse sickness virus (AHSV) VP7 is the major core protein of the virion. Apart from its role in virus assembly, VP7 forms crystalline-like particles during infection and when expressed in insect cells. The aim ...
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Bekker, Shani; Huismans, H. (Henk), 1942-; Van Staden, Vida (MDPI, 2022-07-26)A unique characteristic of the African horse sickness virus (AHSV) major core protein VP7 is that it is highly insoluble, and spontaneously forms crystalline particles in AHSV-infected cells and when expressed in vitro. ...
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Huismans, H. (Henk), 1942-; Van Dijk, A.A.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)The in vitro transcription reaction of bluetongue virus (BTV) is characterized by a core-mediated, temperature-dependent inhibition at high core concentrations and temperatures (Van Dijk & Huismans, 1980; Huismans, Van ...
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Huismans, H. (Henk), 1942-; Erasmus, B.J.; Steyn, P.J.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B. (Published by The Government Printer, Pretoria, 1981)The bluetongue virus (BTV) core particle contains 2 major polypeptides, P3 and P7, and is surrounded by an outer capsid layer that is composed of the 2 major polypeptides, P2 and P5. Analysis of the immune precipitates ...
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Huismans, H. (Henk), 1942-; Eksteen, P.A.L.; De Kock, V.E.; De Lange, M.; Bigalke, R.D.; Howell, P.G.; Reinecke, R.K.; Walker, Jane B. (Published by the Government Printer, Pretoria, 1972)The stimulation of interferon synthesis by bluetongue virus and by bluetongue virus ribonucleic acid was investigated in order to determine if there is a difference in the mechanism of induction. The molecular mass of the ...
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Huismans, H. (Henk), 1942-; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)Both virus-specific double-stranded and single-stranded ribonucleic acid (RNA) are synthesized during infection. The single-stranded RNA is formed in a large excess of double-stranded RNA and the rate of synthesis is maximal ...
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Huismans, H. (Henk), 1942-; South Africa. Dept. of Agricultural Technical Services; Tustin, R.C.; De Lange, M.; Reinecke, R.K.; Walker, Jane B.; De Kock, V.E. (Pretoria : Government Printer, 1970)Infection of L-cells with bluetongue virus results in inhibition of protein and deoxyribonucleic acid synthesis shortly after infection. No inhibition of ribonucleic acid synthesis is observed before 7 hours after infection. ...
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Stassen, Liesel; Huismans, H. (Henk), 1942-; Theron, Jacques (Springer, 2011-04)The VP5 outer capsid protein of African horse sickness virus (AHSV) is cytotoxic when expressed in Spodoptera frugiperda (Sf-9) cells. Secondary structure analysis of the VP5 amino acid sequence of AHSV-9 identified two ...
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Huismans, H. (Henk), 1942-; Howell, P.G.; De Kock, V.E.; De Lange, M.; Bigalke, R.D.; Howell, P.G.; Reinecke, R.K.; Walker, Jane B. (Pretoria : The Government Printer, 1973)Isolates of ³H-labelled messenger RNA of a number of different bluetongue virus serotypes were hybridized with saturating amounts of denatured ³²P-labelled double-stranded RNA of different serotypes. These cross-hybridization ...
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Huismans, H. (Henk), 1942-; University of Pretoria. Dept. of Genetics (University of Pretoria, 1990-03-15)Research training at a University takes place by means of student participation in research programmes designed to develop the necessary skills, understanding and motivation that are required to undertake research. The ...
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Huismans, H. (Henk), 1942-; Bremer, C.W.; Barber, T.L.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)Purified epizootic haemorrhagic disease virus (EHDV) was shown to contain 10 double-stranded RNA segments and a double-layered protein capsid with 4 major and 4 minor polypeptides. The virus differed from bluetongue virus ...
Now showing items 1-20 of 27