Kombucha multimicrobial community under simulated spaceflight and martian conditions

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dc.contributor.author Podolich O.
dc.contributor.author Zaets I.
dc.contributor.author Kukharenko O.
dc.contributor.author Orlovska I.
dc.contributor.author Khirunenko L.
dc.contributor.author Sosnin M.
dc.contributor.author Haidak A.
dc.contributor.author Shpylova S.
dc.contributor.author Rabbow E.
dc.contributor.author Skoryk M.
dc.contributor.author Kremenskoy M.
dc.contributor.author Demets R.
dc.contributor.author Kozyrovska N.
dc.contributor.author De Vera J.-P.
dc.contributor.upauthor Reva, Oleg N.
dc.date.accessioned 2017-07-17T05:45:10Z
dc.date.issued 2017-05
dc.description.abstract Kombucha microbial community (KMC) produces a cellulose-based biopolymer of industrial importance and a probiotic beverage. KMC-derived cellulose-based pellicle film is known as a highly adaptive microbial macrocolony - a stratified community of prokaryotes and eukaryotes. In the framework of the multipurpose international astrobiological project "BIOlogy and Mars Experiment (BIOMEX)," which aims to study the vitality of prokaryotic and eukaryotic organisms and the stability of selected biomarkers in low Earth orbit and in a Mars-like environment, a cellulose polymer structural integrity will be assessed as a biomarker and biotechnological nanomaterial. In a preflight assessment program for BIOMEX, the mineralized bacterial cellulose did not exhibit significant changes in the structure under all types of tests. KMC members that inhabit the cellulose-based pellicle exhibited a high survival rate; however, the survival capacity depended on a variety of stressors such as the vacuum of space, a Mars-like atmosphere, UVC radiation, and temperature fluctuations. The critical limiting factor for microbial survival was high-dose UV irradiation. In the tests that simulated a 1-year mission of exposure outside the International Space Station, the core populations of bacteria and yeasts survived and provided protection against UV; however, the microbial density of the populations overall was reduced, which was revealed by implementation of culture-dependent and culture-independent methods. Reduction of microbial richness was also associated with a lower accumulation of chemical elements in the cellulose-based pellicle film, produced by microbiota that survived in the post-test experiments, as compared to untreated cultures that populated the film. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.embargo 2018-05-30
dc.description.librarian hj2017 en_ZA
dc.description.sponsorship This study was supported by National Academy of Sciences of Ukraine (grant 47/2012-15). The pre-flight programs EVTs and SVTs for the EXPOSE-R2 mission were supported by the European Space Agency. en_ZA
dc.description.uri http://www.liebertpub.com/overview/astrobiology/99 en_ZA
dc.identifier.citation Podolich O., Zaets I., Kukharenko O., Orlovska I., Reva O., Khirunenko L., Sosnin M., Haidak A., Shpylova S., Rabbow E., Skoryk M., Kremenskoy M., Demets R., Kozyrovska N., and de Vera J.-P.. Astrobiology. May 2017, 17(5): 459-469. https://doi.org/10.1089/ast.2016.1480. en_ZA
dc.identifier.issn 1531-1074 (print)
dc.identifier.issn 1557-8070 (online)
dc.identifier.other 10.1089/ast.2016.1480
dc.identifier.uri http://hdl.handle.net/2263/61357
dc.language.iso en en_ZA
dc.publisher Mary Ann Liebert en_ZA
dc.rights © 2017 Mary Ann Liebert, Inc. All rights reserved. en_ZA
dc.subject Bacterial cellulose en_ZA
dc.subject Biofilm en_ZA
dc.subject Biology and mars experiment (BIOMEX) en_ZA
dc.subject Biosignature en_ZA
dc.subject Kombucha multimicrobial community (KMC) en_ZA
dc.title Kombucha multimicrobial community under simulated spaceflight and martian conditions en_ZA
dc.type Postprint Article en_ZA


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