dc.contributor.author |
Korb, Vanessa C.
|
|
dc.contributor.author |
Phulukdaree, Alisa
|
|
dc.contributor.author |
Lalloo, Umesh G.
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|
dc.contributor.author |
Chuturgoon, Anil A.
|
|
dc.contributor.author |
Moodley, Devapregasan
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|
dc.date.accessioned |
2016-10-12T12:23:00Z |
|
dc.date.issued |
2016-07 |
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dc.description.abstract |
T-helper (Th) 17 cells are a pro-inflammatory subset of CD4+ effector T-cells critical in mucosal immunity. Imbalances in Th17 cell proportion have been implicated in the pathogenesis of several diseases; however, this has not been adequately explored in tuberculosis (TB) and human immunodeficiency virus (HIV) co-infection. Since Th17 cells are predominantly mucosally associated, we assessed Th17 proportion and associated microenvironment in pleural effusions from patients co-infected with TB/HIV. Our results show that TB+HIV+ pleurisy results in significantly reduced frequency of CD4+IL-17+RORC+STAT3+ Th17 cells compared to TB−HIV−ex vivo (p = 0.0054) and was confirmed in conditioned media studies in vitro (p = 0.0001). This was not associated with alterations in Th17 polarising cytokines IL-6, IL-21 and IL-23 or changes in Th17 signature cytokines IL-17A and F. However, the mRNA expression of Th17 signalling molecules, IL-6 (p = 0.0022), IL-6R (p = 0.0247), IL-1β (p = 0.0022) and signal transducer and activator (STAT) 3 (p = 0.0022) were significantly upregulated. Notably, TB+HIV+ pleural fluid contained significantly higher concentrations of IL-1β (p = 0.0008), IL-22 (p = 0.0115), IL-31 (p = 0.0210), TNF-α (p = 0.0251) and IFN-γ (p = 0.0026) than TB−HIV− pleural fluid ex vivo. Taken together, this suggests a reduced portion of Th17 lymphocytes in TB/HIV pleurisy is independent of locally mediated cytokine polarisation. |
en_ZA |
dc.description.department |
Physiology |
en_ZA |
dc.description.embargo |
2017-07-31 |
|
dc.description.librarian |
hb2016 |
en_ZA |
dc.description.sponsorship |
The National
Research Foundation, KwaZulu-Natal Research Institute for Tuberculosis
and HIV and College of Health Sciences, University of
KwaZulu-Natal. |
en_ZA |
dc.description.uri |
http://intl.elsevierhealth.com/journals/tube |
en_ZA |
dc.identifier.citation |
Korb, VC, Phulukdaree, A, Lalloo, UGA, Chuturgoon, AA & Moodley, D 2016, 'TB/HIV pleurisy reduces Th17 lymphocyte proportion independent of the cytokine microenvironment', Tuberculosis, vol. 99, pp. 92-99. |
en_ZA |
dc.identifier.issn |
1472-9792 (print) |
|
dc.identifier.issn |
1873-281X (online) |
|
dc.identifier.other |
10.1016/j.tube.2016.05.001 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/57127 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2016 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Tuberculosis. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Tuberculosis, vol. 99, pp. 92-99, 2016. doi : 10.1016/j.tube.2016.05.001. |
en_ZA |
dc.subject |
Co-infection |
en_ZA |
dc.subject |
Th17 |
en_ZA |
dc.subject |
IL-17 |
en_ZA |
dc.subject |
Tuberculosis (TB) |
en_ZA |
dc.subject |
Human immunodeficiency virus (HIV) |
en_ZA |
dc.subject |
IL-1β |
en_ZA |
dc.title |
TB/HIV pleurisy reduces Th17 lymphocyte proportion independent of the cytokine microenvironment |
en_ZA |
dc.type |
Postprint Article |
en_ZA |