1977 Onderstepoort Journal of Veterinary Research, Volume 44, 1977

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CONTENTS

Volume 44: Number 1

Disseminated protothecosis in a dog Imes, GD, Lloyd, JC & Brightman, MP 1

Volatile fatty acid metabolism in sheep. 2. Correlation between the volatile fatty acid production and concentration in the rumen during the course of a feeding cycle Van der Walt, JG 7

Oviposition and incubation in Boophilus decoloratus (Koch, 1844) (Acarina : Ixodidae) Londt, JGH 13

The life cycle of the two-host tick Rhipicephalus evertsi evertsi Neumann, 1897, under laboratory conditions (Acarina : Ixodidae) Londt, JGH & Van der Bijl, EB 21

Gamma-glutamyl transpeptidase activity in sheep serum : normal values and an evaluation of its potential for detecting liver involvement in experimental lupinosis Malherbe, WD, Kellerman, TS, Kriek, NPJ & Haupt, WH 29

Negative staining of a non-haemadsorbing strain of African swine fever virus Els, HJ & Pini, A 39

Research notes:

Effect of levamisole on immunity to Corynebacterium pseudotuberculosis in mice and sheep Cameron, CM 47

First report of Fascioloides magna (Bassi, 1875) in South Africa Boomker, JDF & Dale-Kuys, JC 49

Volume 44: Number 2

The taxonomic position of Clostridium botulinum Type C Jansen, BC & Knoetze, PC 53

Parasites of domestic and wild animals in South Africa. I. Oestrus ovis in sheep Horak, IG 55

Parasites of domestic and wild animals in South Africa. II. Oestrus ovis in goats Horak, IG & Butt, MJ 65

The separation of some volatile fatty acids on a "Sephadex" partition chromatogram Van der Walt, JG 69

Studies on Schistosomiasis. 9. Pathology of the bovine urinary tract Bartsch, RC & Van Wyk, JA 73

The arterial blood supply of the cervical vertebrae of the ox (Bos Taurus L.) Smuts, MMS 95

Studies on Parafilaria bovicola Tubangui, 1934. 2. Chemotherapy and pathology Viljoen, JH & Boomker, JDF 107

Parasites of domestic and wild animals in South Africa. III. Oestrus spp. and Gedoelstia hässleri in the blesbok Horak, IG & Butt, MJ 113

Hydrops amnii in sheep associated with hydranencephaly and arthrogryposis with Wesselsbron disease and Rift Valley fever viruses as aetiological agents Coetzer, JAW & Barnard, BJH 119

Volume 44: Number 3

Neurosecretion in Ornithodoros savignyi (Audouin) (Ixodoidea : Argasidae). The distribution of neurosecretory cells in the brain Evans, AA & Solomon, KR 127

A revision of the genus Impalaia Mönnig, 1924 Boomker, JDF 131

Sheep erythrocyte and bluetongue virus antibody responses of spleen cell cultures from mice Oellermann, RA & Carter, P 139

Ixodes (Afrixodes) neitzi, n. sp. (Acarina : Ixodidae) from the mountain reedbuck in South Africa Clifford, CM, Walker, JB & Keirans, JE 143

Immunosuppression in new-born lambs Broekman, J, Eksteen, PAL & Verwoerd, DW 151

The fine structure of intra-erythrocytic stages of Babesia bigemina Potgieter, FT & Els, HJ 157

An accurate sensitive and reproducible method for colorimetric estimation of free fatty acids in plasma De Villiers, S, Van der Walt, JG & Procos, J 169

Cryopreservation of the infective larvae of the common nematodes of ruminants Van Wyk, JA, Gerber, HM & Van Aardt, WP 173

Research notes:

Neurological symptoms in a cat following vaccination with high egg passage Flury rabies vaccine of chicken embryo origin Barnard, BJH, Geyer, HJ & De Koker, WC 195

A rapid method for differentiating between the infective larvae of Oesophagostomum columbianum and Chabertia ovina Van Wyk, JA 197

Volume 44: Number 4

The immunological response to intact and dissociated bluetongue virus in mice Oellermann, RA & Carter, P 201

The pathology of Rift Valley fever. I. Lesions occurring in natural cases in new-born lambs Coetzer, JAW 205

Light and electron microscopic observations on development of Babesia bigemina in larvae, nymphae and non-replete females of Boophilus decoloratus Potgieter, FT & Els, HJ 213

The venous drainage of the cervical vertebrae of the ox (Bos taurus L.) Smuts, MMS 233

Black grain mycetoma (maduromycosis) in horses Boomker, JDF, Coetzer, JAW & Scott, DB 249

Failure to induce in rabbits effective immunity to a mixed infection of Fusobacterium necrophorum and Corynebacterium pyogenes with a combined bacterin Cameron, CM & Fuls, WJP 253

Amaurosis in sheep resulting from treatment with rafoxanide Prozesky, L & Pienaar, JG 257

Parasites of domestic and wild animals in South Africa. IV. Helminths in sheep on irrigated pasture on the Transvaal Highveld Horak, IG & Louw, JP 261

Transplantation of cultured jaagsiekte (sheep pulmonary adenomatosis) cells into athymic nude mice Verwoerd, DW, Mayer-Sharrer, E & Du Plessis, JL 271

Research note:

Diarrhoea in pigs induced by rotavirus Prozesky, L & Theodoridis, A 275

Author index 279

Subject index 281



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    Subject index
    (Pretoria : The Government Printer, 1977) Anonymous; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
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    Author index
    (Pretoria : The Government Printer, 1977) Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
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    Diarrhoea in pigs induced by rotavirus
    (Pretoria : The Government Printer, 1977) Theodoridis, A.; Prozesky, Leon; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Electron microscope examination of the faeces of scouring pigs revealed virus particles which were morphologically indistinguishable from rotavirus (reo-like), a virus associated with diarrhoea in neonatal pigs (Leece, King & Mock, 1976). This is the first record of this virus in the Republic of South Africa.
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    A revision of the genus Impalaia Mönnig, 1924
    (The Government Printer, Pretoria, 1977) Boomker, Jacob Diederik Frederik; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    A revision of the genus lmpalaia Monnig, 1924 forms the subject of this report. Besides the type species, lmpalaia tuberculata Monnig, 1924, there are 2 valid species, viz., I. nudicollis Monnig, 1931and I. okapiae (Van den Berghe, 1937). I. tuberculata longispiculata (Wetzel & Fortmeyer, 1960), I. somaliensis (Crovieri, 1929) and I. aegyptiaca Soliman, 1956 are synonymous with I. tuberculata. I. taurotragi (LeRoux, 1936) appears to be an aberrant form of/. nudicollis. A parasite/host checklist is included.
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    Transplantation of cultured jaagsiekte (sheep pulmonary adenomatosis) cells into athymic nude mice
    (The Government Printer, Pretoria, 1977) Du Plessis, J.L.; Verwoerd, Daniel Wynand; Meyer-Scharrer, Elizabeth; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Epithelial cells of the 15 .4 line, which were originally established from the adenomatous lung of a jaagsiekte case and which had been cultured in vitro for 22 generations, were injected subcutaneously into athymic nude mice. A slow-growing tumour which soon became cystic was established in each case. The cysts rapidly increased in size as a result of the accumulation of a slightly turbid secretion containing aggregates of tumour cells which rapidly refilled the cysts after the fluid had been withdrawn. Cultures were readily re-established from these cells and a chromosomal analysis proved that the tumour consisted of sheep cells. An epithelial cell lining, very similar to that found in the adenomatous lung alveoli of typical jaagsiekte, could be demonstrated histologically.
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    Light and electron microscopic observations on the development of Babesia bigemina in larvae, nymphae and non-replete females of Boophilus decoloratus
    (Pretoria : The Government Printer, 1977) Potgieter, F.T.; Els, H.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    In Boophilus decoloratus infected by transovarian passage with B. bigemina, primary schizogony occurred as a continuous repetitive process in all 3 stages of the tick's life cycle spent on the host. The primary schizonts and the large merozoites (= vermicules) produced by them were observed in the gut epithelium, haemocytes, muscles and peritracheal cells. Secondary schizogony which led to the formation of small merozoites (= infective forms) occurred mainly in the salivary glands, but was also observed in the cortex of the synganglion. Mature small merozoites were observed in nymphal and adult ticks only. An infective stabilate was prepared from nymphae collected on Day 14 and Day 15 post larval infestation. The infections resulting from intravenous injection of the stabilate had a prepatent period of 8 days.
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    Parasites of domestic and wild animals in South Africa. IV. Helminths in sheep on irrigated pasture on the Transvaal Highveld
    (The Government Printer, Pretoria, 1977) Horak, Ivan Gerard; Louw, J.P.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    The seasonal incidence of helminth infestation in sheep on newly established irrigated pastures was determined by the slaughter of pairs of tracer lambs exposed for periods of approximately 33 days from October 1968 - July 1970 and of sets of 3 lambs from March 1971-May 1973. It took several months for the infestation to become established on the pasture, but thereafter, of the various species present, Haemonchus contortus was most prevalent. In general, peak burdens of this species were recovered from January-May or June, while marked inhibition of larval development was evident from April - August. Ostertagia circumcincta usually occurred in increased numbers from April-October, and larval development was inhibited during July and August. Although the sizes of the burdens varied considerably from year to year, Trichostrongylus spp. Were present mainly from April-August.
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    Amaurosis in sheep resulting from treatment with rafoxanide
    (Pretoria : The Government Printer, 1977) Prozesky, Leon; Pienaar, J.G.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Amaurosis occurred in sheep on various farms in the Republic of South Africa after treatment with rafoxanide. Histopathological examination revealed a status spongiosus of varying severity in the central nervous system in all the cases, having a predilection for certain areas such as the periventricular area of the lateral ventricles, optic tracts, lateral geniculates and optic fasciculi. The retina was the only ocular tissue affected and lesions observed in the retina included necrosis of nerve cells in the ganglionic layer. In chronic cases of amaurosis this layer showed a complete absence of nerve cells. The possible pathogenesis of the lesions and their differentiation from those found in certain plant toxicoses are discussed.
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    The pathology of Rift Valley fever. I. Lesions occurring in natural cases in new-born lambs
    (The Government Printer, Pretoria, 1977) Coetzer, Jacobus A.W.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    A widespread epizootic of Rift Valley fever occurred in the Republic of South Africa and South West Africa during 1974-75. This is a report on the gross pathology of 34 new-born lambs and the histopathology of 93 new-born lambs that died during this outbreak. The liver was affected in every case and showed the most pronounced lesions. The organ was grossly enlarged in most cases, with scattered greyish-white necrotic foci 1-2 mm in diameter and haemorrhages of varying size throughout. Haemorrhages were also frequently seen in the mucosa of the abomasum. The massive diffuse necrosis of hepatocytes (pannecrosis) associated with well-demarcated foci of primary coagulative necrosis, present in 100% of the cases examined, was characteristic of the histopathology of the new-born lamb. Bile thrombi were noticed in the livers of 31% of the lambs and intranuclear inclusions in 49% of the cases. The diagnostic significance of the microscopic liver lesions is discussed. Focal necrosis and haemorrhages were frequently seen in the adrenal cortex while generalized destruction of lymphocytes in the lymph nodes and spleen occurred in many of the animals. In addition, the following hitherto undescribed or previously not well-documented lesions are recorded: (i) mineralization of single or groups of necrotic hepatocytes in 62% of the livers: (ii) pyknosis and karyorrhexis of the cellular elements in the glomeruli and a hyalinized appearance of many of these affected glomeruli; and (iii) necrosis of the tips of the villi in the small intestine in some of the animals.
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    Neurosecretion in Ornithodoros savignyi (Audouin) (Ixodoidea : Argasidae). The distribution of neurosecretory cells in the brain
    (The Government Printer, Pretoria, 1977) Evans, Aileen A.; Solomon, K.R.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    The arrangement of the brain and peripheral nerves in Ornithodoros savignyi (Audouin) is similar to that of other argasid and ixodid ticks. Histological studies, using a specialized staining technique (aldehyde fuchsin), have shown 15 groups of neurosecretory cells in the cortex of the brain.
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    Failure to induce in rabbits effective immunity to a mixed infection of Fusobacterium necrophorum and Corynebacterium pyogenes with a combined bacterin
    (Pretoria : The Government Printer, 1977) Cameron, Colin McKenzie; Fuls, W.J.P.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Rabbits were immunized with alum-precipitated, oil adjuvant and an untreated bacterin composed of F. necrophorum and C. pyogenes. Immunized rabbits were challenged intradermally with a mixture of F. necrophorum and C. pyogenes. Initially a low level of initial transient resistance could be demonstrated but a solid immunity could not be established.
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    Black grain mycetoma (maduromycosis) in horses
    (Pretoria : The Government Printer, 1977) Coetzer, Jacobus A.W.; Boomker, Jacob Diederik Frederik; Scott, D.B.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Black grain mycetoma occurred in two horses, one a cross-bred pony and the other a Thoroughbred racehorse. Lesions were limited to wart-like growths in one case and a deep-seated girth gall in the other. In both cases the granules in the affected tissues were similar in pigmentation and structure to those produced by Curvularia geniculata in dogs. Since all the specimens were received in formalin, the causative fungus could not be isolated. These are the first cases of maduromycosis in animals to be recorded in South Africa.
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    The immunological response to intact and dissociated bluetongue virus in mice
    (Pretoria : The Government Printer, 1977) Carter, P.; Oellermann, R.A.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Antigenic fractions of bluetongue virus were separated by ultracentrifugation in Tris-buffered CsCl gradients at pH 6, 7 or 8 and the bluetongue virus polypeptide composition of the bands isolated from these gradients was monitored by polyacrylamide gel slab electrophoresis. The immunological response to these fractions in mice was determined by a haemolytic plaque-forming cell assay, using sheep erythrocytes onto which intact bluetongue virus was adsorbed as lytic indicator cells. Isolated outer layer bluetongue virus polypeptide 2, from gradients at pH 6, and polypeptides 2 and 5, from gradients at pH 7, produced a strong primary JgM plaque-forming cell response. The subviral particles of density 1,39 g.cmֿ ֿֿֿ³ and the bluetongue virus core particles of density 1,42 g.cmֿ ֿ³ also stimulated an IgM response at least as strong as that to intact bluetongue virus of density 1,38 g.cmֿֿ ֿ³. The isolated bluetongue virus fractions therefore appear to maintain their immunogenic integrity as effectively as those of intact bluetongue virus. The pattern of the immune response to bluetongue virus type 4 is similar to that of type 10
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    Hydrops amnii in sheep associated with hydranencephaly and arthrogryposis with Wesselsbron disease and Rift Valley fever viruses as aetiological agents
    (Pretoria : The Government Printer, 1977) Coetzer, Jacobus A.W.; Barnard, B.J.H.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    During the 1974/75 lambing season numerous reports were received from various parts of the Republic of South Africa and South West Africa of severe abdominal distension in ewes after vaccination with the attenuated Rift Valley fever and/or attenuated Wesselsbron disease vaccine. The ewes were vaccinated at different stages of gestation in spite of recommendations to the contrary, the syndrome being especially obvious in ewes immunized with one or both of these vaccines during the first trimester of pregnancy. In some of the flocks hydrops amnii was recorded in as many as 15% of the ewes. Many of the ewes so affected showed a prolonged gestation of up to 6-7 months and, towards the end of gestation, were unable to rise or walk. They eventually died of ketosis, hypostatic pneumonia and complications due to dystocia. The foetuses examined were malformed and larger than normal with a mass of 3,6-6,7 kg. They usually showed arthrogryposis, brachygnathy inferior, hydranencephaly, hypoplasia or segmental aplasia of the spinal cord and neurogenic muscular atrophy. The amnion contained 8,0-18,0 l of amniotic fluid, the endometrium was oedematous, and cystic tube-like dilatations, 1-10 mm in diameter, filled with a clear fluid, were scattered in the endometrium. No definite conclusions as to the aetiology of the syndrome could be drawn from serological tests performed on the ewes, lambs or foetuses. Preliminary experimental work confirmed previous observations that the attenuated Wesselsbron disease vaccine virus is responsible for this syndrome and that the wild-type virus is also implicated. In addition, the attenuated Rift Valley fever vaccine virus was found to the responsible for arthrogryposis and hydranencephaly without hydrops amnii and for micrencephaly and arthrogryposis associated with hydrops amnii in the ewe.
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    A rapid method for differentiating between the infective larvae of Oesophagostomum columbianum and Chabertia ovina
    (The Government Printer, Pretoria, 1977) Van Wyk, Jan Aucamp; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    When freshly-collected, exsheathed infective larvae of Oesophagostomum columbianum and Chabertia ovina were frozen in liquid nitrogen and subsequently thawed, conspicuous vesicles appeared in the intestinal cells of the larvae. The mean number of vesicles, which differed in size according to the species was 20 for O. columbianum and 32 for C. ovina.
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    The venous drainage of the cervical vertebrae of the ox (Bos taurus L.)
    (The Government Printer, Pretoria, 1977) Smuts, M.M.S. (Malie Marie Sophie), 1933-; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    The venous drainage of the cervical vertebrae of the ox was studied in 16 animals (Friesland, Jersey and Guernsey cross-breeds), ranging from near full-term foetuses to adults. The extra- and intraosseous veins of the cervical vertebrae are described and illustrated. The nutrient vessels are derived from the vertebral, deep cervical, occipital and internal jugular veins. In addition to the dorsal and ventral external venous plexuses, a lateral external venous plexus is described. The intraosseous veins correspond in the main to the arteries. They are described and illustrated with reference to the available literature. In both young and adult animals anastomoses between epi- and metaphysial vessels are reported. The role of the internal ventral vertebral venous system as a collateral pathway is stressed and comments are made on its possible functional role.
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    Parasites of domestic and wild animals in South Africa. I. Oestrus ovis in sheep
    (The Government Printer, Pretoria, 1977) Horak, Ivan Gerard; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Separate groups of 3 oestrid-free lambs were exposed to infestation on irrigated pasture for periods of approximately 33 days each over 30 months, and on dry-land pasture for approximately 42 days over a period of 18 months. With some exceptions, the lambs slaughtered from October – June were found to be infested with Oestrus ovis while, with one exception, those slaughtered from July - September were free. A minimum of 4 sheep’s heads, obtained weekly over 24 months from the Pretoria Municipal Abattoir, was examined for infestation. Of a total of 542 heads examined, 73, 4% were infested, having a mean burden of 15, 2 larvae. Mean larval burdens were slightly greater in hornless than in horned sheep, in Dorper-type than in Merino-type sheep, and in lambs than in sheep with 2 or more permanent incisors. The largest larval burdens were recovered from sheep slaughtered during May and June and the smallest during September and October. The greatest number of 1st instar larvae were recovered during May and June and the smallest during September, but those recovered during the latter month were the largest. With one exception, mature larvae which pupated after 21 March or before 16 August failed to hatch as viable flies. Those which pupated after 16 August hatched as flies after a pupal stage of approximately 50 days and the first flies to hatch were invariably recovered during the first 2 weeks of October. The pupal stage decreased to approximately 25 days during December and January and increased again to approximately 50 days for flies hatching during May. No flies hatched between 18 May and 1 October. The following life cycle for Oestrus ovis is suggested: sheep are repeatedly infested from October - June; thereafter infestation survives in the sheep’s' heads until August, mainly as 1st instar larvae, then as pupae and larvae until fresh infestation takes place during October.
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    The taxonomic position of Clostridium botulinum Type C
    (The Government Printer, Pretoria, 1977) Jansen, B.C.; Knoetze, P.C.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Experimental evidence is produced to justify abandoning the practice of subdividing Clostridium botulinum Type C into type Cα and type Cβ.
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    Parasites of domestic and wild animals in South Africa. III. Oestrus spp. and Gedoelstia hässleri in the blesbok
    (The Government Printer, Pretoria, 1977) Horak, Ivan Gerard; Butt, M.J.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    Four blesbok culled in the Rietvlei Nature Reserve, Pretoria, District during May 1972 were found to harbour large burdens of 1st instar Oestrus spp. larvae and from 16-37 3rd instar larvae of Oestrus macdonaldi. They were also infested with large numbers of 1st instar Gedoelstia hässleri larvae but only 2 harboured 3rd stage larvae of this species. During an 18-month period 34 blesbok were culled in pairs in the Percy Fyfe Nature Reserve, Potgietersrus District. These antelope harboured peak numbers of 1st instar Oestrus spp. Larvae during February, July and December but few if any during early October. Third instar Oestrus variolosus larvae were generally recovered from July-February and those of 0. macdonaldi during July 1972 and from May-September 1973. Some 1st instar larvae of these flies appeared to undergo a pulmonary migration before returning to the naso-pharyngeal area to mature. The pupal period of 0. variolosus varied from 67 days during the spring to 35 days during the summer. G. hässleri larvae reached peak numbers from October 1972-January 1973 and during May and June 1973. The lowest numbers were recovered from August-October 1973. Recovery and measurement of 1st ins tar larvae indicated that they either undergo an ocular-cranial or ocular-vascular-pulmonary migration before reaching the naso-pharyngeal area. Pupal periods varied from 46 days for flies hatching during October to 22 days for those hatching during December.
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    Neurological symptoms in a cat following vaccination with high egg passage Flury rabies vaccine of chicken embryo origin
    (Pretoria : The Government Printer, 1977) Barnard, B.J.H.; Geyer, H.J.; De Koker, W.C.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; De Kock, V.E.
    The development of neurological symptoms in a cat following vaccination with the high egg passage Flury rabies vaccine and the subsequent isolation of a virus with characteristics consistent with the criteria for distinguishing the high egg passage Flury strain of rabies virus are described.